Loading Control Antibodies
Loading controls are used in Western blots for two main purposes:
1) To estimate relative amounts of total protein loaded in each lane.
(This information is needed to determine if
the difference in levels of the protein of interest in different
samples is due to loading and transfer errors or to real changes in
protein expression.) 2) To troubleshoot the Western blotting procedure. The proteins that are usually used as loading controls are known to be constitutively and stably expressed at high levels in almost all tissues and cells. They include housekeeping proteins, such as GAPDH, tubulin, and actin.
GenScript is proud to provide a wide selection of polyclonal and monoclonal loading control antibodies for GAPDH, α-tubulin, α-actin, β-actin for several species. HRP- and biotin-conjugated versions of these antibodies are also available.
To simplify the loading control detection process, GenScript offers a series of One-Step WesternTM Multiplex Kits. These kits allow simultaneous detection of both the protein of interest and the loading control in a simple one-hour procedure.
Example:
Comparison of Detection Sensitivity of Anti-β-Actin Monoclonal Antibodies from GenScript and Company X
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Primary Antibody:
A. 0.1 μg/ml Mouse Anti-β-Actin mAb (GenScript, A000702)
B. 0.1 μg/ml Mouse Anti-β-Actin mAb (Company X)
Secondary Antibody:
Goat Anti-Mouse IgG (H&L) [HRP] pAb (GenScript, 1:10,000, A00160)
The signal was developed with LumisensorTM HRP Substrate Kit (GenScript, L00221V500)
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Product Overview:
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