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Control Antibodies

Control Antibodies

Control Antibodies

Isotype Control Antibodies

The selection of an appropriate isotype control is key for flow cytometry experiments. Isotype control antibodies that have no relevant specificity can distinguish non-specific "background" staining from specific antibody staining. Three main factors contribute to the levels of background staining associated with a primary antibody: a) binding to Fc receptors on target cells, b) non-specific protein interactions with cellular proteins, lipids, or carbohydrates, and c) cell autofluorescence. All of these factors vary greatly depending on the target cell type and the isotype of the primary antibody. Therefore, in order to accurately determine the level of specific staining by the primary antibody, isotype controls need to be matched to the specific primary antibodies being used. GenScript offers a series of isotype control antibodies that can be used in your research.

Loading Control Antibodies

Loading control proteins are known to be constitutively and stably expressed at high levels in almost all tissues and cells. Housekeeping proteins, such as GAPDH, tubulin, and actin, represent some of the well-known loading control proteins.

Loading control antibodies are used in Western blotting for two main purposes:

  1. To estimate relative amounts of total protein loaded in each lane. (This information is needed to determine if the difference in levels of the protein of interest in different samples is due to loading and transfer errors or to real changes in protein expression.)
  2. To troubleshoot the Western blotting procedure.

GenScript offers a wide selection of polyclonal and monoclonal loading control antibodies for GAPDH, α-tubulin, and β-actin for several species. HRP- and biotin-conjugated versions of these antibodies are also available.

  • Product Details
  • Examples

Ordering Information:

All 40μg antibodies are under 40% off promotion!


Isotype Control Antibodies

Cat. No.
Product Name
Size
Price
Quantity
A01010 Chicken IgY Control (Whole Molecule), Purified
4 mg
$69.00
A01009 Goat IgG Control (Whole Molecule), Purified
4 mg
$69.00
A01383 Human IgG Control [FITC] (Whole Molecule), Purified
0.500 mg
$69.00
A01006 Human IgG Control(Whole Molecule), Purified
4 mg
$69.00
A01007 Mouse IgG control (Whole Molecule), Purified
1 mg
$69.00
A01381 Mouse IgG Control [FITC] (Whole Molecule), Purified
0.500 mg
$69.00
A01008 Rabbit IgG Control (Whole Molecule), Purified
4 mg
$69.00
A01730 Mouse IgG2b control (Whole Molecule), Purified
1 mg
$69.00
A01382 Rabbit IgG Control [FITC] (Whole Molecule), Purified
0.500 mg
$69.00

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Loading Control Antibodies

Cat. No.
Product Name
Size
Price
Quantity
A00702-40 THETM beta Actin Antibody, mAb, Mouse
40 μg
$69.00 $41.4
A00702-100 THETM beta Actin Antibody, mAb, Mouse
100 μg
$175.00
A00702-200 THETM beta Actin Antibody, mAb, Mouse
200 μg
$311.00
A00730-40 THETM beta Actin Antibody [HRP], mAb, Mouse
40 μg
$74.00 $44.4
A00730-200 THETM beta Actin Antibody [HRP], mAb, Mouse
200 μg
$333.00
A01546 THETM beta Actin Antibody [Biotin], mAb, Mouse
100 μg
$335.00
A01410-40 THETM alpha Tubulin Antibody, mAb, Mouse
40 μg
$108.00 $64.8
A01410-100 THETM alpha Tubulin Antibody, mAb, Mouse
100 μg
$225.00
A01490 THETM alpha Tubulin Antibody [HRP], mAb, Mouse
100 μg
$335.00
A01545 THETM alpha Tubulin Antibody [Biotin], mAb, Mouse
100 μg
$335.00
A00885-40 α-Actin-1 Antibody, pAb, Rabbit
40 μg
$79.00 $47.4
A00885-200 α-Actin-1 Antibody, pAb, Rabbit
200 μg
$275.00
A01203-40 β-Tubulin III Antibody, pAb, Rabbit
40 μg
$79.00 $47.4
A01203-200 β-Tubulin III Antibody, pAb, Rabbit
200 μg(5x40μg)
$275.00
A01622-40 GAPDH Antibody, mAb, Mouse
40 μg
$79.00 $47.4
A00191-40 GAPDH Antibody, pAb, Goat
40 μg
$90.00 $54
A00191-100 GAPDH Antibody, pAb, Goat
100 μg
$198.00
A00192-100 GAPDH Antibody [HRP], pAb, Goat
100 μg
$218.00
A00915-40 GAPDH Antibody [Biotin], pAb, Goat
40 μg
$87.00 $52.2
A00915-200 GAPDH Antibody [Biotin], pAb, Goat
200 μg(5x40μg)
$300.00
A01419-40 VDAC1 Antibody, pAb, Rabbit
40 μg
$79.00 $47.4
A01419-200 VDAC1 Antibody, pAb, Rabbit
200 μg(5x40μg)
$275.00
A01717-40 β-tubulin Antibody (2G7D4), mAb, Mouse
40 μg
$79.00 $47.4
A01717-200 β-tubulin Antibody (2G7D4), mAb, Mouse
200 μg(5x40μg)
$275.00

Examples:

  1. Sensitivity Comparison


Primary Antibody:
        A. 0.1 μg/ml THE™ β-Actin mAb(Mouse)(GenScript, A00702)
        B. 0.1 μg/ml β-Actin mAb (Mouse) (Company X)
Secondary Antibody:
         Goat Anti-Mouse IgG (H&L) [HRP] pAb (GenScript, 1:10,000, A00160)
        The signal was developed with Lumisensor™ HRP Substrate Kit (GenScript, L00221V500)

  2. Immunofluenrescence Analysis


Primary Antibody:
        0.1 μg/ml THE™ β-Tubulin mAb (GenScript, A01410)
Secondary Antibody:
        Fluorescein conjugated affinity purified anti-Mouse IgG (H&L) (Goat) (Rockland, 1:1,000, 610-102-121)

  3. Fluorescence Western Blotting


Primary Antibody:
        0.1 μg/ml GAPDH Antibody, pAb, Goat (GenScript, A00191)
Lane 1-8:
         5.0, 2.5,1.25, 0.62, 0.31, 0.16, 0.08 and 0.04 μg of Hela cell lysate
        The signal was developed with One-Step Western™ Fluorescent Kit (GenScript, L00397)

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