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GPCR Membrane Preparation

G-protein independent binding assays are preferred methods in assaying GPCR activation especially in primary screening. Utilizing high-expression GPCR stable cell lines, GenScript offers cell membrane preparations validated by receptor binding assays.

With large-scale cell culture capabilities, GenScript also offers custom membrane preparation services upon your specifications.

Key Features

Examples

Saturation Binding for ADRA1A
Fig A. 0.5 μg of membranes prepared from CHO-K1 cells stably expressing ADRB2 receptors were incubated with indicated concentrations of [3H]CGP-12177 in the absence (total binding) or presence of 1000-fold excess unlabeled ICI 118551 (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.
 
5-HT7 Receptor Saturation Binding
Fig B. 0.5 μg of membranes prepared from 293 cells stably expressing 5-HT7 receptors were incubated with indicated concentrations of [3H]5-HT in the absence (total binding) or presence of 1000-fold excess unlabeled 5-HT (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.

Membrane Preparation Service and Product:

    Custom Membrane Preparation Service

Sample Requirement
If you want to produce membranes utilizing your stable cell line, please submit 2 vials cells (3 x 106 cells/vial).
Timeline:
< 10 mg: 4 weeks;
< 50 mg: 5 weeks.
> 50 mg: Please inquire.
Delivery:
Standard aliquote: 1 mg/vial. For custom aliquot, Please inquire.

Cat. No. Service
Price (USD)
SC1486
Custom Membrane Preparation Service
Quotation

    Membrane Preparation Products

For bulk order, please inquire.


Related Custom Products and Services


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