Cell Lines and Membranes
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Custom Membrane Production Service

GPCR & Ion Channel Membrane Preparation

G-protein independent binding assays are preferred methods in assaying GPCR activation especially in primary screening. Utilizing high-expression GPCR & ion channel stable cell lines, GenScript offers cell membrane preparations validated by receptor binding assays.

With large-scale cell culture capabilities, GenScript also offers custom membrane preparation services upon your specifications.

Key Features:

  • Consistent quality: All membrane preparations are from stable cell lines.
  • Receptor yield: High receptor expression stable cell lines are used.
  • Quality control: Every batch of membrane preparations is validated by radioligand binding assay.
  • Large-scale production: 1010 cells are available for membrane production within one month.

Examples:

Saturation Binding for ADRA1A
Fig A. 0.5 μg of membranes prepared from CHO-K1 cells stably expressing ADRB2 receptors were incubated with indicated concentrations of [3H]CGP-12177 in the absence (total binding) or presence of 1000-fold excess unlabeled ICI 118551 (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.
 
5-HT7 Receptor Saturation Binding
Fig B. 0.5 μg of membranes prepared from 293 cells stably expressing 5-HT7 receptors were incubated with indicated concentrations of [3H]5-HT in the absence (total binding) or presence of 1000-fold excess unlabeled 5-HT (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.

Membrane Preparation Service and Product:

    Custom Membrane Preparation Service

Sample Requirement
If you want to produce membranes utilizing your stable cell line, please submit 2 vials cells (3 x 106 cells/vial).
Timeline:
< 10 mg: 4 weeks;
< 50 mg: 5 weeks.
> 50 mg: Please inquire.
Delivery:
Standard aliquote: 1 mg/vial. For custom aliquot, Please inquire.

Cat. No. Service
Price (USD)
SC1486
Custom Membrane Preparation Service
Quotation

    Membrane Preparation Products

Cat. No. Name Family Receptor Size Price (USD) Selected
293/hERG Potassium, voltage-gated hERG (Kv11.1)
1 vial
$320.00
CHO-K1/M2/Gα15 Acetylcholine (muscarinic) M2 / CHRM2
1 vial
$320.00
CHO-K1/M3 Acetylcholine (muscarinic) M3 / CHRM3
1 vial
$320.00
CHO-K1/M4/Gα15 Acetylcholine (muscarinic) M4 / CHRM4
1 vial
$320.00
CHO-K1/M5 Acetylcholine (muscarinic) M5 / CHRM5
1 vial
$320.00
CHO-K1/ADRA1A Adrenoceptors ADRA1A
1 vial
$320.00
CHO-K1/ADRA1B Adrenoceptors ADRA1B
1 vial
$320.00
CHO-K1/ADRA1D Adrenoceptors ADRA1D
1 vial
$320.00
CHO-K1/ADRB2/Gα15 Adrenoceptors ADRB2
1 vial
$320.00
CHO-K1/D1 Dopamine D1
1 vial
$320.00
CHO-K1/D2S/Gα15 Dopamine D2S
1 vial
$320.00
CHO-K1/D5/Gα15 Dopamine D5
1 vial
$320.00
CHO-K1/OPRD1/Gα15 Opioid OPRD1
1 vial
$320.00
CHO-K1/OXTR Oxytocin OT / OXTR
1 vial
$320.00
CHO-K1/5-HT1A Serotonin 5-HT1A / HTR1A
1 vial
$320.00
293/5-HT2C Serotonin 5-HT2C / HTR2C
1 vial
$320.00
293/5-HT7/CRE-Luc Serotonin 5-HT7 / HTR7
1 vial
$320.00
CHO-K1/V1B Vasopressin V1B / AVPR1B
1 vial
$320.00
CHO-K1/V2 Vasopressin V2 / AVPR2
1 vial
$320.00
For bulk order, please inquire.

Related Custom Products and Services:


Email: bioassay@genscript.com
Phone: 1-877-436-7274 (Toll-Free), 1-732-885-9188
Fax: 1-732-210-0262, 1-732-885-5878