Microwave Peptide Synthesis Technology

Microwaves can be very useful when it comes to improving product yields and speeding up reaction rates. At present, microwaves are used to improve solid-phase peptide synthesis, to facilitate chemical coupling, and to ease the chemical modification of proteins.

GenScript has developed and applied microwave technology to its peptide-manufacturing pipeline, enabling us to secure the highest success rate in the industry, over 95%.

Microwave Technology Enhances Chemical Coupling in Peptide Chain Assembly

Advantages of Microwave Technology:

In traditional peptide synthesis, amino acids are added sequentially to a growing peptide chain immobilized on a solid support. The chain is extended through repeated cycles of coupling and deprotection: For example, each dangling N-terminal amine of a solid-supported peptide is coupled to an amino acid featuring a protected N-terminal amine. The deprotection step exposes the extended peptide's N-terminal amine, to which further amino acids can be added.

GenScript microwave-enhanced peptide synthesis offers researchers the following benefits:

• Increased reaction rate and reduced synthesis time: The use of microwave heating in peptide synthesis can markedly reduce the time required to complete each cycle of coupling and deprotection.
• Improved peptide yields: The use of microwave energy can improve peptide yields by preventing unwanted aggregation.
• Decreased cost: Microwave technology significantly decreases the cost of peptide synthesis by enhancing chemical reaction rate and reducing the quantity of chemicals required for synthesis.
• Stitching capability: Researchers can use microwave energy to put preassembled peptides together.
• Improved compatibility: Microwave energy can also facilitate site-specific protein modification.

More about GenScript peptide synthesis