| Product Name |
DYKDDDDK Lysates |
Description |
This product consists of 293 cells transfected with a DYKDDDDK-tagged minigene, 60 kDa, 100 μg/100 ul. Human embryonic kidney (HEK) 293 cells were originally derived from human embryonic kidney tissue and subsequently shown to be a useful cell type for the production of adenoviruses, other vial vectors, and effectively glycosylated human recombinant proteins. Such cells are therefore used for virology studies and transfected to express recombinant proteins for studies in a number of different fields. |
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| Reference |
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Linggi B, Carpenter G. ERBB-4 s80 intracellular domain abrogates ETO2-dependent transcriptional repression. J. Biol. Chem. Jun 2006.
Mori K, et al. Oligomerizing potential of a focal adhesion LIM protein Hic-5 organizing a nuclear-cytoplasmic shuttling complex. J. Biol. Chem. May 2006.
Kobayashi A, et al. Efflux of sphingomyelin, cholesterol and phosphatidylcholine by ABCG1. J. Lipid Res. May 2006.
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Storage |
For long term storage, please keep the product at -70°C. During shipping, the product needs to be kept in an environment between -20°C and 4°C. Repeated freeze and thaw cycles should be avoided. |
Application |
Recommended dilutions: DYKDDDDK Peptide lysate is a lysate produced from 293 cells transfected with a 60 kDa DYKDDDDK-tagged minigene. It is delivered ready to load on SDS-PAGE gels for western blotting. 10 µg per lane is recommended for mini gels. |
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