| Product Name |
Streptavidin-HRP |
Description |
Streptavidin is a protein that has similar binding properties to avidin. It is isolated from Streptomyces avidinii. Streptavidin has a molecular weight of 60 KD and four subunits. Each subunit can bind one molecule of biotin. Biotin is a 244-dalton water-soluble vitamin. Streptavidin has an extremely high binding affinity: (Kd=10-15) for biotin.
Streptavidin-HRP is steptavidin coupled to horseradish peroxidase (HRP). This highly sensitive detector conjugate binds to biotinylated primary and secondary antibodies in enzyme immunoassays, immunoblotting, immunohistochemistry, flow cytometry,and hybridoma screening systems. This conjugate also binds to nucleic acids labeled with biotinylated nucleotide analogs in Southern and northern blots. |
|
Concentration |
1 mg/ml in PBS (pH 7.4) containing 10 mg/ml BSA and 0.01% thimerosal |
Storage |
The reagent may be stored at 4°C for short periods of one month or less. For longer periods,aliquot and store at -20°C . Avoid repeated freeze/thaw cycles. If slight precipitation occurs upon prolonged storage, clarify the solution by centrifugation before use. |
Application |
Working concentrations for specific applications should be determined by titration assay. Appropriate concentrations will be affected by several factors including primary antibody, antigen amount, sensitivity of detection method, temperature, and incubation time. The following dilution ranges are recommended starting points for this product:
Dot blot: 1:2,000-1:10,000
Western blot: 1:2,000-1:20,000
ELISA: 1:50,000-1:200,000
Others: User-optimized |
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PRODUCTINFO: 20061221032815 (PDF)