| Product Name |
293/H1 Stable Cell Line |
Full Name |
Human Recombinant H1 Histamine Receptor Stable Cell Line |
|
Species |
Human |
Description |
The H1 histamine receptor is expressed primarily in the lungs, vasculature, and brain. The H1 mediates the contraction of smooth muscles, neurotransmission in the central nervous system, the release of catecholamine from adrenal medulla, and increases in capillary permeability due to contraction of terminal venules. H1's role in inflammatory responses makes its antagonist suitable for treating allergies. |
Host Cell |
293 |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
DMEM, 10% FBS, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assays for H1 receptor |
Application Examples |
Intracellular calcium response from 293 cells stably expressing human H1 histamine receptors and from untransfected control cells. Cells were loaded with Calcium-4 then stimulated with the indicated concentrations of histamine. Calcium responses were recorded on a FlexStation plate reader. Data represent the average +/- standard deviation of triplicate determinations. |
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1 |
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TECHNICAL MANUAL: 20080905022527 (PDF)
