WI38

*This product has been discontinued!*

WI-38 cell (human lung fibroblasts) lysate is prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, and 5 µg/ml of leupeptin). Cell debris are removed by centrifugation. Protein concentration is determined via Bio-Rad protein assay. The cell lysate is boiled for five minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, and 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

Cat. No.	M0015
Size	Buy in bulk

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Overview Properties Applications

Note

Cell line: WI-38 (Human lung fibroblast, normal) Growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, and 10% FBS

Overview

Species

Human

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Properties

Purity	Whole cell lysate
Tissue	lung
Concentration	The product is supplied at 100 μg/100 μl. whole cell lysate, in 1 x SDS sample buffer containing 5% b-mercaptoethanol.
Storage	Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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Applications

WI-38 cell lysate is delivered ready-to-load. It is ideal for SDS-PAGE and Western blotting. We recommend 10 - 20 µg per lane isrecommended for mini-gels.

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