GenScript - molecule: WI38

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Product Name	WI38	Figures
Species	Human	References: Probin V, et al. Busulfan selectively induces celluar senescence but not apoptosis in WI38 fibroblasts via a P53-independent but ERK-P38 MAPK-dependent mechanism. Pharmacol. Exp. Ther. Aug 2006. Boojar MM and Goodarzi F. Cytotoxicity and the levels of oxidative stress parameters in WI38 cells following 2 macrocyclic crown ethers treatment. Clin. Chim. Acta. Feb 2006; 364(1-2): 321-327. Semov A, et al. Alterations in TNF- and IL-related gene expression in space-flown WI38 human fibroblasts. FASEB J. Jun 2002; 16(8): 899-901.
Tissue	lung
Description	WI-38 cell (human lung fibroblasts) lysate is prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, and 5 µg/ml of leupeptin). Cell debris are removed by centrifugation. Protein concentration is determined via Bio-Rad protein assay. The cell lysate is boiled for five minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, and 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Concentration	The product is supplied at 100 μg/100 μl. whole cell lysate, in 1 x SDS sample buffer containing 5% b-mercaptoethanol.
Note	Cell line: WI-38 (Human lung fibroblast, normal) Growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, and 10% FBS
Storage	Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity	Whole cell lysate
Application	WI-38 cell lysate is delivered ready-to-load. It is ideal for SDS-PAGE and Western blotting. We recommend 10 - 20 µg per lane isrecommended for mini-gels.