DNA Marker |
Immunology Reagents |
Protein Marker |
RNA Marker |
Lysates |
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| Product Name |
HT29 |
Figures |
Species |
Human |
References:
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Ye YJ, et al. Antisense to CDK4 inhibits the growth of human colon cancer cells HT29. Zhonghua Yi Xue Za Zhi. Mar 2006; 86(12): 846-849.
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Kobori M, et al. 5alpha,8alpha-Epidioxy-22E-ergosta-6,9(11),22-trien-3beta-ol from an edible mushroom suppresses growth of HL60 leukemia and HT29 colon adenocarcinoma cells. Biol. Pharm. Bull. Apr 2006; 29(4): 755-759.
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Tissue |
Gastrointestinal |
Description |
HT29 (human colon adenocarcinoma grade II cell line) whole cell lysate. |
Concentration |
Concentration: 100 μg/100 μl
Storage buffer: Whole lysate in 1 X SDS 5% b-mercaptoethanol
|
Volume |
10 - 20 µg per lane isrecommended for mini gel |
Storage |
Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles
|
Background |
HT29 cells that produce carcinoembryonic antigen (CEA) and are human intestinal epithelial cells which produce the secretory component of immunoglobulin A (IgA). These cells are used for tumourigenicity studies. The lysate is a good positive control for keratin 18, keratin 20 (several antibodies in our catalog), the protooncogene cyclin D1 (several antibodies in our catalog). HT 29 cell lysate is prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, and 5 µg/ml of leupeptin). Cell debris is removed by centrifugation. Protein concentration is determined via Bio-Rad protein assay. The cell lysate is boiled for five minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%sodium dodecylsulfate, and 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
|
Application |
HT 29 cell lysate is delivered ready-to-load on SDS-PAGE for western blotting. 10 - 20 µg per lane isrecommended for mini-gels. |
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