| Product Name |
CHO-K1/ADRB2/Gα15 Stable Cell Line |
Full Name |
Human Recombinant ADRB2 Adrenoceptors Stable Cell Line |
|
Species |
Human |
Description |
β-Adrenergic receptors (β-ARs) are members of the superfamily G-protein-coupled receptors that are stimulated by naturally occurring catecholamines, epinephrine, and norepinephrine. As part of the sympathetic nervous system, β-ARs have been shown to have important roles in cardiovascular, respiratory, metabolic, central nervous system, and reproductive functions. Three distinct β-AR subtypes have been identified (β1-AR, β2-AR, and β3-AR). All three of these β-AR subtypes are believed to signal by coupling to the stimulatory G-protein Gsα which leads to the activation of adenylyl cyclase and accumulation of the second messenger cAMP. β1-ARs mediate cardiac stimulation, β2-ARs mediate smooth muscle relaxation in the peripheral vasculature and respiratory system, and β3-AR has been shown to have important roles in adipose tissue and gastrointestinal tract. In studies using subtype-selective agonists and antagonists in the human heart, β2-AR stimulation leads to the activation of adenylyl cyclase and contributes to both inotropic and chronotropic responses. |
Host Cell |
CHO-K1/Gα15 |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for ADRB2 receptor |
Application Examples |
Figure 1. Isoproterenol-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRB2/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a ADRB2 receptor agonist, Isoproterenol. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Isoproterenol (Mean ± SD, n = 2). The EC50 of Isoproterenol on ADRB2 co-expressing with Gα15 in CHO-K1 cells was 0.05 nM. The S/B of Isoproterenol on ADRB2 co-expressing with Gα15 in CHO-K1 cells was 5. |
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TECHNICAL MANUAL: 20091127040418 (PDF)
