Figure 1. Isoproterenol-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRB2/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a ADRB2 receptor agonist, Isoproterenol. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Isoproterenol (Mean ± SD, n = 2). The EC50 of Isoproterenol on ADRB2 co-expressing with Gα15 in CHO-K1 cells was 0.05 nM. The S/B of Isoproterenol on ADRB2 co-expressing with Gα15 in CHO-K1 cells was 5.
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