| Product Name |
CHO-K1/EP2/Gα15 Stable Cell Line |
Full Name |
Human Recombinant EP2 Prostanoid Receptor Stable Cell Line |
|
Species |
Human |
Description |
Prostaglandin (PG) E2 exerts its actions by acting on a group of G-protein-coupled receptors (GPCRs). There are four GPCRs responding to PGE2 designated subtypes EP1, EP2, EP3, and EP4 and multiple splicing isoforms of the subtype EP3. The EP subtypes exhibit differences in signal transduction, tissue localization, and expression regulation. Studies have identified that EP2 mediates many processes, such as facilitating ovulation and fertilization, suppressing dendritic cell differentiation, and promoting amyloid-β formation in Alzheimer's disease. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery
|
Application |
Functional assay for EP2 receptor |
Application Examples |
Figure 1. PGE2-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/EP2/Gα15 and CHO-K1. The cells were loaded with Calcium-4 prior to stimulation with an EP2 receptor agonist, PGE2. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of PGE2 (Mean ± SD, n = 2). The EC50 of PGE2 on EP2 co-expressing with Gα15 in CHO-K1 cells was 21 nM. The S/B of PGE2 on EP2 co-expressing with Gα15 in CHO-K1 cells was 6. |
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TECHNICAL MANUAL: 11169_20091030024217.PDF (PDF)
Document-EXAMPLE: 11171_20091030024411.JPG (JPG)