| Product Name |
Human ADRA1B Receptor Membrane Preparation |
Species |
Human |
| Documents |
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 TECHNICAL MANUAL: 11208_20091111222006.PDF (PDF) |
| Document-EXAMPLE: 11217_20091112003606.JPG (JPG) |
| Document-EXAMPLE: 11216_20091112003549.JPG (JPG) |
| Figures |
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| Reference |
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Wang J (1997) A Mutation in the Hamster a1B-Adrenergic Receptor that Differentiates Two Steps in the Pathway of Receptor Internalization. Mol. Pharmacol. 52: 306-312
Klijn K et al. (1991) Renal a.-Adrenergic Receptor Subtypes: MDCK-D1 Cells, But Not Rat Cortical Membranes Possess a Single Population of Receptors. Mol. Pharmacol. 39: 407-413.
Veenstra DMJ et al. (1992) Determination of acz-adrenoceptor subtype selectivity by [3H]-prazosin displacement studies in guinea-pig cerebral cortex and rat spleen membranes. Br. J. Pharmacol. 107: 202-206
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Description |
The α1-adrenergic receptor (AR) family consists of three closely related gene products (α1A, α1B, and α1D). They mediate the actions of norepinephrine (NE) and epinephrine in sympathetically innervated tissues and brain. α1-ARs belong to the G protein-coupled receptor family and consist of single polypeptide chains that are predicted to form seven transmembrane spanning domains. With similar pharmacological and signaling properties, α1-AR subtypes act through Gq/11 proteins to activate phospholipase C, increase inositol 1,4,5-trisphosphate production, and increase intracellular Ca2+. ADRA1B functions in diverse settings include vasoconstriction and myocardial contractility, neuronal dopaminergic responses, dendritic cell migration and inflammatory responses, as well as neuroendocrine regulation of fertility. |
Host Cell |
CHO-K1/ADRA1B |
Concentration |
1 mg/ml in 50 mM HEPES, 0.1 mM EDTA, 10 % glycerol |
Storage |
Store at -80°C |
Quality Control |
Bmax and Kd are determined using radioligand saturation binding assays (Figure 1).
Expression Level (Bmax): 0.62 pmol/mg membrane protein.
Kd for [3H]Prazosin: 0.06 nM
|
Application |
Binding assays for ADRA1B receptor |
 |
1 |
Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.39/bp, with advanced FREE codon optimization tool and seamless cloning technology. |
2 |
PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days. |
3 |
Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package. |
4 |
Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50. |
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Document-EXAMPLE: 11217_20091112003606.JPG (JPG)
Document-EXAMPLE: 11216_20091112003549.JPG (JPG)