Human D2S Receptor Membrane Preparation

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Product Name

Human D2S Receptor Membrane Preparation

Species

Human

Documents
TECHNICAL MANUAL: 11300_20091127000326.PDF (PDF)
Document-EXAMPLE: 12389_20100401212934.JPG (JPG)
Document-EXAMPLE: 12390_20100401213024.JPG (JPG)
Figures
Reference
Hayes G et al. (1992). Structural subtypes of the dopamine D2 receptor are functionally distinct: expression of the cloned D2A and D2B subtypes in a heterologous cell line. Mol. Endocrinol. 6(6):920-6. Grandy DK et al. (1989). Cloning of the cDNA and gene for a human D2 dopamine receptor. Proc. Natl. Acad. Sci. USA. 86:9762; McGonigle P et al. (1984). A comprehensive method for the quantitative determination of dopamine receptor subtypes. Ann. N. Y. Acad. Sci. 430:77-90.

Description

The short form of D2 (D2S) and the long form (D2L) are two isoforms that contribute differentially to dopamine signaling in both prefrontal cortex and striatum. The D2 dopamine receptor, short form (D2S) has been shown to stimulate phospholipase D (PLD) activity independent of the activation of phospholipase C (PLC) activity in GH4 derived cells stably transfected with the D2S receptor. Agonist activation of D2s has been shown to mediate the inhibition of growth in the same cell line.

Host Cell

CHO-K1/D2s/Gα15

Concentration

1 mg/ml in 50 mM HEPES, 0.1 mM EDTA, 10 % glycerol

Storage

Store at -80°C

Quality Control

B_max and K_d are determined using radioligand saturation binding assays (Figure 1).
Expression Level (B_max): 3.26 pmol/mg membrane protein.
K_d for [³H]Spiperone: 0.16 nM

Application

Binding assay for D2S receptor


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