Human 5-HT2C Receptor Membrane Preparation

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*This product is under development*

Product Name

Human 5-HT2C Receptor Membrane Preparation

Description

5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal, and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). 5HT2C is expressed in the brain and spinal cord, especially the choroid plexus. 5HT2C receptor agonists may have important clinical value in the treatment of mental and eating disorders, such as depression, panic anxiety, OCD, bulimia, and obesity. GenScript's cloned human 5-HT2C expressing cell line is generated in the CHO-K1 host.

Documents
TECHNICAL MANUAL: 11212_20091111222218.PDF (PDF)
Document-EXAMPLE: 11214_20091111223241.JPG (JPG)
Document-EXAMPLE: 11215_20091111223511.JPG (JPG)
Figures
Reference
Im WB et al. (2003) Positive Allosteric Modulator of the Human 5-HT2C Receptor. Mol. Pharmacol. 64:78–84 Davis K et al. (2006) Serotonin 5-HT2C Receptor Homodimer Biogenesis in the Endoplasmic Reticulum. J. Biol. Chem. 281:27109-27116. Ni YG et al. (1997) Blockage of 5HT2C serotonin receptors by fluoxetine (Prozac). Proc. Natl. Acad. Sci. USA 94: 2036-2040.

Host Cell

HEK293/5-HT2C

Concentration

1 mg/ml in 50 mM HEPES, 0.1 mM EDTA, 10 % glycerol

Storage

Store at -80°C

Quality Control

B_max and K_d are determined using radioligand saturation binding assays (Figure 1).
Expression Level (B_max): 6.76 pmol/mg membrane protein.
K_d for [³H]5-Hydroxytryptamine: 6.89 nM

Application

Binding assay for 5-HT2C receptor


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