| Product Name |
Human V1B Receptor Membrane Preparation |
Species |
Human |
| Documents |
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 TECHNICAL MANUAL: 11366_20091127011200.PDF (PDF) |
| Document-EXAMPLE: 12527_20100415025052.JPG (JPG) |
| Document-EXAMPLE: 12528_20100415025124.JPG (JPG) |
| Figures |
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| Reference |
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Sugimoto T et al. (1994) Molecular cloning and functional expression of a cDNA encoding the human V1b vasopressin receptor. J Biol Chem. 269(43):27088-92.
Tahara A, et al. (1998) Pharmacological characterization of the human vasopressin receptor subtypes stably expressed in Chinese hamster ovary cells. Br J Pharmacol. 125(7):1463-70.
Serradeil-Le Gal C et al. (2007) Biological characterization of rodent and human vasopressin V1b receptors using SSR-149415, a nonpeptide V1b receptor ligand. Am J Physiol Regul Integr Comp Physiol. 293(2):R938-49. Epub 2007 May 23.
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Description |
The antidiuretic hormone arginine vasopressin (AVP) receptors are G protein-coupled receptors which consists of at least three types: V1A (vascular/hepatic) and V1B (anterior pituitary) receptors, which act through phosphatidylinositol hydrolysis to mobilize intracellular Ca2+, and V2 (kidney) receptor, which is coupled to adenylate cyclase. V1B receptors are expressed in anterior pituitary where they mediate the release of ACTH. Its peripheral actions, such as antidiuresis, contraction of vascular smooth muscle, and stimulation of hepatic glycogenolysis are well characterized. |
Host Cell |
CHO-K1/V1B |
Concentration |
2 mg/ml in 50 mM HEPES, 0.1 mM EDTA, 10 % glycerol |
Storage |
Store at -80°C |
Quality Control |
Bmax and Kd are determined using radioligand saturation binding assays (Figure 1).
Expression Level (Bmax): 1.31 pmol/mg membrane protein.
Kd for [3H](Arg8)Vasopressin: 0.28 nM
|
Application |
Binding assay for V1B receptor |
 |
1 |
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2 |
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3 |
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4 |
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Document-EXAMPLE: 12527_20100415025052.JPG (JPG)
Document-EXAMPLE: 12528_20100415025124.JPG (JPG)