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M00405
 Human ADRA1D Receptor Membrane Preparation
1 vial
$320
Product Name Human ADRA1D Receptor Membrane Preparation
Species
 Human
Documents
TECHNICAL MANUAL: 11653_20100104031319.PDF (PDF)
Document-EXAMPLE: 11657_20100104033926.JPG (JPG)
Document-EXAMPLE: 11658_20100104034417.JPG (JPG)
Figures
Reference
Kenny B et. al. (1995) Characterization of an alpha 1D-adrenoceptor mediating the contractile response of rat aorta to noradrenaline. Br. J. Pharmacol. 115(6):981-6.

Ko F et. al. (1994) (-)-Discretamine, a selective alpha 1D-adrenoceptor antagonist, isolated from Fissistigma glaucescens. Br. J. Pharmacol. 112(4):1174-80.

Wada T et. al. (1996) Characterization of alpha 1-adrenoceptor subtypes in rat spinal cord. Eur. J. Pharmacol. 312(2):263-6.
Description
 The α1-adrenergic receptor (AR) family consists of three closely related gene products (α1A, α1B, and α1D) that mediate the actions of norepinephrine (NE) and epinephrine in sympathetically innervated tissues and brain. α1-ARs belong to the G protein-coupled receptor family and consist of single polypeptide chains predicted to have seven transmembrane spanning domains. With similar pharmacological and signaling properties, α1-AR subtypes act through Gq/11 proteins to activate phospholipase C, increase both inositol 1,4,5-trisphosphate production and intracellular Ca2+. Once activated by binding, α1-ARs initiate the cellular pathways leading to the regulation of physiological effects, including blood pressure maintenance, glucose metabolism, renal sodium reabsorption, and cardiac inotropy.
Host Cell
 CHO-K1/ADRA1D
Concentration
 1 mg/ml in 50 mM HEPES, 0.1 mM EDTA, 10 % glycerol
Storage
 Store at -80°C
Quality Control
 Bmax and Kd are determined using radioligand saturation binding assays (Figure 1).
Expression Level (Bmax): 0.73 pmol/mg membrane protein.
Kd for [3H]Prazosin: 0.19 nM
Application
 Binding assay for ADRA1D receptor
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