| Product Name |
Human ADRB2 Receptor Membrane Preparation |
Species |
Human |
|
Description |
β-Adrenergic receptors (β-ARs) are members of the superfamily G-protein-coupled receptors that are stimulated by naturally occurring catecholamines, epinephrine, and norepinephrine. As part of the sympathetic nervous system, β-ARs have been shown to have important roles in cardiovascular, respiratory, metabolic, central nervous system, and reproductive functions. Three distinct β-AR subtypes have been identified (β1-AR, β2-AR, and β3-AR). All three of these β-AR subtypes are believed to signal by coupling to the stimulatory G-protein Gsα which leads to the activation of adenylyl cyclase and accumulation of the second messenger cAMP. β1-ARs mediate cardiac stimulation, β2-ARs mediate smooth muscle relaxation in the peripheral vasculature and respiratory system, and β3-AR has been shown to have important roles in adipose tissue and gastrointestinal tract. In studies using subtype-selective agonists and antagonists in the human heart, β2-AR stimulation leads to the activation of adenylyl cyclase and contributes to both inotropic and chronotropic responses. |
Host Cell |
CHO-K1/ADRB2/Gα15 |
Concentration |
1 mg/ml in 50 mM HEPES, 0.1 mM EDTA, 10 % glycerol |
Storage |
Store at -80°C |
Quality Control |
Bmax and Kd are determined using radioligand saturation binding assays (Figure 1).
Expression Level (Bmax): 31.1 pmol/mg membrane protein.
Kd for [3H]CGP-12177: 0.46 nM
|
Application |
Binding assay for ADRAB2 receptor |
 |
1 |
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|
TECHNICAL MANUAL: 11655_20100104031418.PDF (PDF)
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