| Product Name |
Human 5-HT1A Receptor Membrane Preparation |
Species |
Human |
|
Description |
5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal, and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). 5-HT1A, a subtype of 5-HT receptor, couples to Gi/Go mediates inhibitory neurotransmission. |
Host Cell |
CHO-K1/5-HT1A |
Concentration |
1 mg/ml in 50 mM HEPES, 0.1 mM EDTA, 10 % glycerol |
Storage |
Store at -80°C |
Quality Control |
Bmax and Kd are determined using radioligand saturation binding assays (Figure 1).
Expression Level (Bmax): 0.75 pmol/mg membrane protein.
Kd for [3H]5-Hydroxytryptamine: 0.84 nM
|
Application |
Binding assay for 5-HT1A receptor |
 |
1 |
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TECHNICAL MANUAL: 11905_20100223001235.PDF (PDF)
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