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M00409
 Human M2 Receptor Membrane Preparation
1 vial
$320
Product Name Human M2 Receptor Membrane Preparation
Species
 Human
Documents
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TECHNICAL MANUAL: 12307_20100319040402.PDF (PDF)
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Figures
Reference
Cembala TM et al. (1998) Interaction of neuromuscular blocking drugs with recombinant human m1-m5 muscarinic receptors expressed in Chinese hamster ovary cells. Br. J. Pharmacol. 125(5):1088-94.

Mary H et al. (1995) Human muscarinic receptors expressed in A9L and CHO cells: activation by full and partial agonists. Br J Pharmacol. 114(6):1241-9.

Schwarz RD et al. (1993) Characterization of muscarinic agonists in recombinant cell lines. Life Sci. 52(5-6):465-72.

Description
 Muscarinic acetylcholine receptors belong to a superfamily of seven-TM-domain receptors that interact with G-proteins to initiate intracellular responses. Five muscarinic receptor subtypes have been identified and named from M1 to M5. M2 muscarinic receptors act via a Gi type receptor, which causes a decrease in cAMP in the cell, generally leading to inhibitory-type effects.
The M2 muscarinic receptors are located in the heart, where they act to slow the heart rate down to normal sinus rhythm after stimulatory actions of the sympathetic nervous system, by slowing the speed of depolarization. They also reduce contractile forces of the atrial cardiac muscle, and reduce conduction velocity of the atrioventricular node (AV node). However, they have no effect on the contractile forces of the ventricular muscle.
Host Cell
 CHO-K1/M2/Gα15
Concentration
 1 mg/ml in 50 mM HEPES, 0.1 mM EDTA, 10 % glycerol
Storage
 Store at -80°C
Quality Control
 Bmax and Kd are determined using radioligand saturation binding assays (Figure 1).
Expression Level (Bmax): 1.92 pmol/mg membrane protein.
Kd for [3H]N-Methylscopolamine: 0.36 nM
Application
 Binding assay for M2 receptor
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