Human D5 Receptor Membrane Preparation

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Product Name

Human D5 Receptor Membrane Preparation

Species

Human

Documents
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TECHNICAL MANUAL: 12278_20100311195709.PDF (PDF)
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Figures
Reference
Sunahara RK et al. (1991) Cloning of the gene for a human dopamine D5 receptor with higher affinity for dopamine than D1. Nature. 18;350(6319):614-9. Karpa KD et al. (1999) N-linked glycosylation is required for plasma membrane localization of D5, but not D1, dopamine receptors in transfected mammalian cells. Mol. Pharmacol. 56(5):1071-8. Montague DM et al. (2001) Quantification of D1B(D5) receptors in dopamine D1A receptor-deficient mice. Synapse. 15;39(4):319-22.

Description

Dopamine is the predominant catecholamine neurotransmitter found in mammalian brain, where it controls a variety of functions including locomotor activity, cognition, emotion, positive reinforcement, food intake, and endocrine regulation. It also plays multiple roles in the periphery as a modulator of cardiovascular function, catecholamine release, hormone secretion, vascular tone, renal function, and gastrointestinal motility. The dopamine receptor family consists of five members, which are classified into two groups, D1-like (D1 and D5) and D2-like (D2, D3, and D4).

Host Cell

CHO-K1/D5/Gα15

Concentration

1 mg/ml in 50 mM HEPES, 0.1 mM EDTA, 10 % glycerol

Storage

Store at -80°C

Quality Control

B_max and K_d are determined using radioligand saturation binding assays (Figure 1).
Expression Level (B_max): 2.36 pmol/mg membrane protein.
K_d for [³H]SCH23390: 0.94 nM

Application

Binding assay for D5 receptor


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