| Product Name |
Human 5-HT7 Receptor Membrane Preparation |
Species |
Human |
| Documents |
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 Document-EXAMPLE: 12391_20100401222452.JPG (JPG) |
| Document-EXAMPLE: 12392_20100401223045.JPG (JPG) |
| TECHNICAL MANUAL: 12393_20100401223550.PDF (PDF) |
| Figures |
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| Reference |
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Gobbi M et al. (1996) Are 5-hydroxytryptamine7 receptors involved in [3H]5-hydroxytryptamine binding to 5-hydroxytryptamine 1nonA-nonB receptors in rat hypothalamus? Mol. Pharmacol. 49(3):556-9.
Clemett DA et al. (1999) Pindolol-insensitive [3H]-5-hydroxytryptamine binding in the rat hypothalamus; identity with 5-hydroxytryptamine7 receptors. Br. J. Pharmacol. 127(1):236-42.
Krobert KA et al. (2001) The cloned human 5-HT7 receptor splice variants: a comparative characterization of their pharmacology, function and distribution. Naunyn Schmiedebergs Arch. Pharmacol. 363(6):620-32.
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Description |
5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal, and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). The 5-HT7 receptor is coupled to Gs (stimulates the production of the intracellular signaling molecule cAMP) and is expressed in a variety of human tissues, particularly in the brain, the gastrointestinal tract, and in various blood vessels. |
Host Cell |
293/5-HT7/CRE-Luc |
Concentration |
1 mg/ml in 50 mM HEPES, 0.1 mM EDTA, 10 % glycerol |
Storage |
Store at -80°C |
Quality Control |
Bmax and Kd are determined using radioligand saturation binding assays (Figure 1).
Expression Level (Bmax): 94.1 pmol/mg membrane protein.
Kd for [3H]5-Hydroxytryptamine: 9.5 nM
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Application |
Binding assay for 5-HT7 receptor |
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Document-EXAMPLE: 12391_20100401222452.JPG (JPG)
Document-EXAMPLE: 12392_20100401223045.JPG (JPG)