Human V2 Receptor Membrane Preparation

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Product Name

Human V2 Receptor Membrane Preparation

Species

Human

Documents
TECHNICAL MANUAL: 12523_20100415021619.PDF (PDF)
Document-EXAMPLE: 12525_20100415024257.JPG (JPG)
Document-EXAMPLE: 12526_20100415024328.JPG (JPG)
Figures
Reference
Lolait SJ, et al. (1992) Cloning and characterization of a vasopressin V2 receptor and possible link to nephrogenic diabetes insipidus. Nature. 357(6376):336-9. Tahara A, et al. (1998) Pharmacological characterization of the human vasopressin receptor subtypes stably expressed in Chinese hamster ovary cells. Br J Pharmacol. 125(7):1463-70. Cotte N, et al. (1998) Identification of residues responsible for the selective binding of peptide antagonists and agonists in the V2 vasopressin receptor. J Biol Chem. 273(45):29462-8.

Description

Arginine vasopressin (AVP) is a cyclic nonapeptide that acts by binding to a family of vasopressin receptors that includes V1a, V1b, and V2 receptors. In particular, V2 receptors are expressed in kidney where vasopressin exerts its antidiuretic action. V1a and V1b couple to Gq and calcium release, whereas V2 couples to Gs. Mutations in V2 result in X-linked nephrogenic diabetes insipidus, a syndrome in which the kidney is unable to concentrate urine, leading to dehydration and hypernatremia. Conversely, elevated levels of AVP lead to hyponatremia in the syndrome of inappropriate antidiuretic hormone secretion (SIADH), congestive heart failure or cirrhosis, and V2 selective antagonists have been developed to treat these conditions.

Host Cell

CHO-K1/V2/Gα15

Concentration

1 mg/ml in 50 mM HEPES, 0.1 mM EDTA, 10 % glycerol

Storage

Store at -80°C

Quality Control

B_max and K_d are determined using radioligand saturation binding assays (Figure 1).
Expression Level (B_max): 8.47 pmol/mg membrane protein.
K_d for [³H](Arg⁸)Vasopressin: 1.62 nM

Application

Binding assay for V2 receptor


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