| Product Name |
CHO-K1/NPFF1/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Neuropeptide FF Receptor 1 Stable Cell Line |
|
Species |
Human |
Description |
The neuropeptide FF receptors are members of the G-protein coupled receptor superfamily of integral membrane proteins which bind the pain modulatory neuropeptides AF and FF. These neuropeptides are thought to be involved in modulation of opioid receptor function in the brain and spinal cord, and can either reduce or increase opioid receptor function depend which tissue they are released in, reflecting a complex role for neuropeptide FF in pain responses. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for NPFF1 receptor |
Application Examples |
Figure 1. RFRP-3-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NPFF1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an NPFF1 receptor agonist, RFRP-3. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of RFRP-3 (Mean ± SD, n = 2). The EC50 of RFRP-3 on NPFF1 co-expressing with Gα15 in CHO-K1 cells was 22 nM. The S/B of RFRP-3 on NPFF1 co-expressing with Gα15 in CHO-K1 cells was 6. |
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