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Cat. No.
 Name
Size   
Price
 Selected
M00440
2 vials
M00441
250 μl
$35
M00442
250 μl
$50
M00443
250 μl
$55
M00444
2 vials
M00446
2 vials
$11600
M00447
2 vials
$11600
M00448
2 vials
$11600
M00449
2 vials
$11600
*This product has been discontinued! *
Product Name PC-3
Species
 Human
Documents
Document-EXAMPLE: 1658_20060714021611.JPG (JPG)
Document-COA: C55241212_M00441_COA.pdf (PDF)
Document-COA: C55251212_M00442_COA.pdf (PDF)
Document-COA: C55261212_M00443_COA.pdf (PDF)
Document-COA: C56191304_M00441_COA.pdf (PDF)
Document-COA: C56201304_M00442_COA.pdf (PDF)
Document-COA: C56211304_M00443_COA.pdf (PDF)
Figures
Reference
Alimirah F, et al. DU-145 and PC-3 human prostate cancer cell lines express androgen receptor: implications for the androgen receptor functions and regulation. FEBS. Lett. Apr 2006; 580(9): 2294-2300.

Kumar S, et al. Anticancer effects of ethanolic neem leaf extract on prostate cancer cell line (PC-3). J. Ethnopharmacol. Apr 2006; 105(1-2): 246-250.

Tissue
 Genito-Urinary
Description
 PC-3 whole cell lysate
Concentration
 Concentration: 100 μg/100 μl.
Storage buffer: whole cell lysate in 1 x SDS sample buffer containing 5% b-mercaptoethanol.
Recommended loading volume
 20 ug per lane.
Storage
 Storage at -20˚C will be stable for three months, storage at –70˚C will be stable for one year.
Background
 PC-3 (Human prostate adenocarcinoma) cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Application
 PC-3 (Human prostate adenocarcinoma) cell lysate is ready to load on SDS-PAGE for Western blotting, 20 ug per lane is recommended for mini gel.
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