| Product Name |
CHO-K1/GPR120/Gα15 Stable Cell Line |
Full Name |
Human Recombinant G-Protein Coupled Receptor 120 Stable Cell Line |
|
Species |
Human |
Description |
GPR120 is a member of the rhodopsin family of G protein-coupled receptors (GPCRs). Recently it has been found that long-chain fatty acids activate the GPR120 and use of a small interference RNA (siRNA) to down-regulate GPR120 expression result in inhibition of adipocyte differentiation. GPR120 is also expressed in the epithelium of the circumvallate papillae. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for GPR120 receptor |
Application Examples |
Figure 1. Linolenic Acid-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GPR120/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a GPR120 receptor agonist, Linolenic Acid. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of Linolenic Acid (Mean ± SD, n = 2). The EC50 of Linolenic Acid on GPR120 co-expressing with Gα15 in CHO-K1 cells was 88 nM. The S/B of Linolenic Acid on GPR120 co-expressing with Gα15 in CHO-K1 cells was 6. |
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