| Product Name |
CHO-K1/CysLT2 Stable Cell Line |
Full Name |
Human Recombinant Cysteinyl Leukotriene Receptor 2 Stable Cell Line |
|
Species |
Human |
Description |
The cysteinyl leukotrienes including leukotriene C4, leukotriene D4, and leukotriene E4, are arachidonic acid derivatives modified by glutathione, Cys-Gly or Cys. Activated mast cells release cysteinyl leukotrienes, which cause smooth muscle contraction, airway constriction, and vascular permeability. The biological effects of the cysteinyl leukotrienes are mediated by two GPCRs, CysLT1 and CysLT2. Cysteinyl leukotriene receptor 2 (CysLTR2) is a Gq-coupled GPCR expressed in moncytes/macrophages, mast cells and eosinophils. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for CysLT2 receptor |
Application Examples |
Figure 1. LTD4-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CysLT2 and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with a CysLT2 receptor agonist, LTD4. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of LTD4 (Mean ± SD, n = 2). The EC50 of LTD4 on CysLT2 in CHO-K1 cells was 2.8 nM. The S/B of LTD4 on CysLT2 in CHO-K1 cells was 12. |
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