| Product Name |
CHO-K1/AMY3/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Amylin Receptor AMY3 Stable Cell Line |
|
Species |
Human |
Description |
Amylin receptors have always been closely associated with calcitonin (CT) receptor. This can be explained because amylin receptors are multimeric complexes, formed by CT receptor interaction with RAMPs. The CT receptor interacts with the three RAMPs, generating multiple subtypes of amylin receptor (AMY1-3).
The AMY3 receptor is a heterodimeric complex of the CT receptor and RAMP3. GenScript's AMY1-expressing stable cell line was made in CHO-K1/Gα15 host cell and optimized for calcium assays.
|
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for AMY3 receptor |
Application Examples |
Figure 1. AM(1-52)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/AMY3/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an AMY3 receptor agonist, AM(1-52). The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of AM(1-52) (Mean ± SD, n = 2). EC50 of AM(1-52) on AMY3 co-expressing with Gα15 in CHO-K1 cells was 150 nM. The S/B of AM(1-52) on AMY3 co-expressing with Gα15 in CHO-K1 cells was 16. |
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TECHNICAL MANUAL: 13700_20110421231141.PDF (PDF)
Document-EXAMPLE: 13702_20110421231245.JPG (JPG)