| Product Name |
C2C12 |
Figures |
Species |
Mouse |
Document:
References:
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Pietrangelo T, et al. Extracellular guanosine-5'triphosphate modulates myogenesis via intermediate Ca2+-activated K+ currents on C2C12 mouse cells. J. Physiol. Feb 2006; 572(3): 721-733.
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Nakai N, et al. Leucine-induced activation of translational initiation is partly regulated by the branched-chain alpha-keto acid dehydrogenase complex in C2C12 cells. Biochem. Biophys. Res. Commun. May 2006; 343(4): 1244-50.
|
Tissue |
muscle |
Description |
C2C12 (Mouse myoblast cell line) Whole cell lysate.
|
Concentration |
Concentration: 100 μg/100 μl.
Storage buffer: Whole cell lysate in 1 X SDS-PAGE sample loading buffer, with 5% b-mercaptoethanol.
|
Note |
Cell line: C2C12 (muscle; myoblast);
Growth media: DMEM & 10% fetal bovine serum.
|
Volume |
C2C12 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. |
Storage |
Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
Background |
C2C12 cells are a useful model to study the differentiation of non-muscle cells to skeletal muscle cells (e.g myosin phosphorylation mechanisms) and express muscle proteins and the androhen receptor (AR).Mouse C2C12 cell lysate was prepared by homogenization in modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
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