24 hour
You are here: Products » Proteins, Expression, Isolation and Analysis » Protein Detection and Immunoassay » ONE-HOUR Western™ Detection Kits » Stripping Solution
GenScript Share
Cat. No.
 Name
Size   
Price
 Selected
M01015-100
 Stripping Solution
100 ml
$30
M01015-500
 Stripping Solution
500 ml
$90
Product Name Stripping Solution
Description
 GenScript's Stripping Solution provides a robust but gentle method for removing primary and secondary antibodies from Western blot developed with chemiluminescent substrates. Our Western blot Stripping Solution allows reprobing of the same membrane for several times using different antibodies, saving time and conserving samples.
Documents
Document-EXAMPLE: 3174_20070409052428.JPG (JPG)
Document-DATASHEET: 3266_20070424023212.PDF (PDF)
Figures
Reference
Key Features
  • Odorless
  • No special incubator needed: the solution works at room temperature
  • Efficient stripping: completely removes most antibodies within 15 minutes
  • Mild formulation: the same membrane can be probed several times over
Note
 1. Membranes that cannot be immediately processed immediately may be stored overnight in PBS or PBST at 4°C.
2. Optimization of incubation time is essential for best results. In general, 15 minutes is enough to remove most antibodies. If high-affinity antibodies prove troublesome, incubate at 37°C for 15 minutes or longer to facilitate complete stripping.
3. To confirm complete removal of the primary antibody, incubate the membrane with the HRP-labeled secondary antibody, and then process just a Western blot.
Storage
 Store at room temperature
1
 Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.39/bp, with advanced FREE codon optimization tool and seamless cloning technology.
2
 PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days.
3
 Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package.
4
 Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50.