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MM0904
Smart His-tagged Protein Standard
100 Lanes
$98.00
Product Name Smart His-Tagged Protein Standard
Description
The Smart His-tagged Potein Standard is a lyophilized mixture of six highly purified recombinant proteins ranging in size from 15 to 115 kDa containing a terminal 6xHis tag. It yields six clear bands of similar intensity on western blots, thus can be used for precise MW determination of proteins on western blot membranes.
Documents
PRODUCTINFO: 20070402024608 (PDF)
MSDS: 20090520063611 (PDF)
Figures
Smart His-tagged Protein Standard Western Blot Result zoom
Reference
Concentration
7.5 μg of each protein in 60 mM Tris-HCl (pH6.8 at 25°C), 0.4% (w/v) SDS, 10 mM DTT, 0.01% (w/v) bromophenol blue and 2% sucrose.
Volume
Add 250 μl of 1x reconstitution buffer, 2.5 μl of the solution is recommended for each lane of a mini-gel.
Storage
Store at -20°C or below. After reconstitution, aliquot and store at -20°C or below.
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