You are here: Products » Proteins, Expression, Isolation and Analysis » Protein Electrophoresis » Protein Standards » Smart His-tagged Protein Standard
GenScript Share
*This product has been discontinued! The improved replacement is  MM0908*
Product Name Smart His-Tagged Protein Standard
Description
The Smart His-tagged Potein Standard is a lyophilized mixture of six highly purified recombinant proteins ranging in size from 15 to 115 kDa containing a terminal 6xHis tag. It yields six clear bands of similar intensity on western blots, thus can be used for precise MW determination of proteins on western blot membranes.
Documents
PRODUCTINFO: 20070402024608 (PDF)
MSDS: 20090520063611 (PDF)
Figures
Smart His-tagged Protein Standard Western Blot Result zoom
Reference
Concentration
7.5 μg of each protein in 60 mM Tris-HCl (pH6.8 at 25°C), 0.4% (w/v) SDS, 10 mM DTT, 0.01% (w/v) bromophenol blue and 2% sucrose.
Volume
Add 250 μl of 1x reconstitution buffer, 2.5 μl of the solution is recommended for each lane of a mini-gel.
Storage
Store at -20°C or below. After reconstitution, aliquot and store at -20°C or below.
1
Gene Synthesis: Custom gene synthesis service starts at as LOW as $0.35/bp, with advanced OptimumGeneTM codon optimization technology and CloneEZ® seamless cloning technology. (starting from $0.29/bp for non-codon optimized human, mouse and rat gene sequences)
2
PCR Cloning and Subcloning: Order GenScript's flexible PCR cloning and subcloning service RIGHT NOW to get fully validated clones delivered in 14 business days.
3
Site-directed Mutagenesis Services: Special mutagenesis bundle starts at $149/mutation. Site-directed Mutagenesis services include point mutations, deletions, and insertions for unparalleled accuracy, unlimited sites and comprehensive, fully validated deliverables.
4
Plasmid DNA Preparation: Endotoxin FREE plasmid DNA preparation services deliver predominantly supercoiling plasmid DNA ranging from 100 ug to 1000 mg starting from $45.