GenScript produces monoclonal antibodies using both ascites and roller bottle culture methods.
The antibodies can be purified by protein A, protein G, and protein L affinity column, or other preferred methods
according to the client's preference. Upon request, the antibodies can be modified enzymatically to produce F(ab')2
or Fab fragments, or conjugated with Biotin, enzymes, or fluorescent dyes.
1. Ascites Production
GenScript ascites production is performed in Balb/c or nude mice. Our typical deliverables include 2-5 ml
ascites per mouse at antibody concentration of 1-10 mg/ml depending on the productivity of individual cell line.
Our typical turnaround time is 4-6 weeks.
GenScript also offers mycoplasma testing, mycoplasma removal, and clonality services for premium ascites production.
Please read the detailed procedures and timelines
of ascites production service.
2. Roller bottle cell culture
Roller bottle cell culture allows for high-quality, reproducible antibody production without the use of animals.
Typically, either low-bovine-IgG fetal bovine sera or serum-free media are used in roller bottle cell culture to
control the possible bovine IgG contamination. Typically, monoclonal antibodies produced in roller bottles are
purified before shipment. Our typical deliverables include 15-50 mg/L monoclonal antibodies depending on the
productivity of individual cell line. Our typical turnaround time is 5-7 weeks. GenScript's roller bottle culture is
in cGMP compliance. We offer mycoplasma testing, mycoplasma removal, and clonality services for optimized production.
Please read the detailed procedures and timelines of roller bottle cell culture service.
3. Antibody Purification
GenScript offers comprehensive antibody purification services, including protein A, protein G, and protein L
affinity column, immuno-affinity column, salt precipitation, and ion-exchange purification. Our scientists
can design and select the most suitable strategy and conditions tailored to each purification project.
The reactivity and purity of the purified antibodies is tested by ELISA /Western blot and SDS-PAGE, respectively.
Our typical deliverables include ELISA and/or Western blot results, SDS-PAGE image, and documentation of the endotoxin
level. Our typical turnaround time is 1-2 weeks.
To request a quotation, please download and complete our Quick Quotation Request.
