Mutagenesis Services

Mutagenesis is central to molecular biology, facilitating research in a variety of fields, including studies of gene regulatory elements, DNA-protein interaction, protein structure/function, enzyme active sites, and novel proteins. GenScript's mutagenesis services, powered by sophisticated gene synthesis technology and high performance GS polymerase, include point mutations, deletions, and insertions. They can be customized to fit any scientific applications.

• Sequence confirmation: The sequence of template DNA will be confirmed by sequencing upon request.
• Primer Design/Synthesis: Primer design and synthesis for mutagenesis are included.
• Mutation Introduction: Mutations are introduced by PCR.
• Mutant Screening: Every mutation is verified by sequencing.
• Mutant DNA Preparation: GenScript can manufacture mutant DNA plasmid up to kilogram scale with required purity and endotoxin levels.

Competitive Advantages:

• Unparalleled Accuracy: GenScript's high performance GS polymerase ensures accuracy of mutants, avoiding creation of unwanted mutations.
• Unlimited Sites: Using our sophisticated mutagenesis technology, GenScript can introduce mutations at any site.
• Mutation on Large Construct: Our technology is optimized to introduce mutations, insertions, and deletions on constructs as large as up to 8 kb (including target gene and its vector).
• Comprehensive Service Package: We offer comprehensive upstream and downstream services, including template sequencing, expression vector construction, and protein expression and purification
• Most Competitive Prices: Our services are at least 50% less expensive than those of our competitors.

Pricing:

*The list price is only for genes less than 1 kb in length.
*For longer gene, if template sequencing is required, an additional $100 per kb fee applies.
*One mutation is defined as any combination of mutations within a 10-base frame.

Sample Submission Requirements:

Please provide following:

• The complete sequence of the template including the target gene and its vector
• Mutation specifications
• A sufficient amount of culture if the starting material is bacterial culture
• Maps and antibiotic resistance of the template and destination vectors
• A 2 µg template DNA if the starting material is DNA

Delivery Specifications:

Our typical turnaround is about 10 business days. Our delivery package includes:

• 4 µg lyophilized destination vector containing the gene
• Stab culture of E. coli transformed with destination vector containing the gene (Only for US customers)
• Sequence chromatograms of the gene (electronic)
• Construct maps (electronic)
• Quality assurance certificate

Quotations and Ordering:

For quotation requests and questions, you may contact us by email, phone, fax. Please download and complete our Mutagenesis Quotation Request Form, send it to us by email or via secure web server.

Orders may be placed by email, phone, fax, or online with either a formal PO or credit card. We recommend that you submit a completed Mutagenesis Quotation Request Form by email or via our secure web sercer. Our customer service representatives are available 24 hours, Monday through Friday to assist you.

Please send your samples with full shipping information and PO number (if applicable) to Mutagenesis Service, GenScript Corp., 120 Centennial Ave., Suite 105, Piscataway, NJ 08854, US.

	Order by email: gene@genscript.com
	Order by phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
	Order by fax: 1-732-210-0262 1-732-885-5878