Home » Gene Synthesis & Molecular Biology Services » Gene Synthesis » Express Cloning & Subcloning

Cloning & Subcloning
custom cloning service quotation

PCR Cloning and Subcloning Services

Are you tired of PCR cloning? GenScript offers cloning services so you can free yourself from routine gene cloning and subcloning and instead devote your energy and time to more creative research. Our molecular biology experts can bundle gene synthesis with cloning into your choice of vector, or you can outsource cloning projects from templates you already have. For your convenience, gene cloning can easily be added onto your gene synthesis order when you request a quote online.

Our new Express Cloning service offers cloning into your choice of free vectors for mammalian and bacterial expression – for an unbeatable price of only $49 and 2-day turnaround added to your gene synthesis order.
See the full list of Free Express Cloning Vectors you can choose from »

Our Subcloning bundled with gene synthesis (SC1017) and Custom PCR Cloning (SC1622) services are highly flexible to meet any specific project requirements. Simply tell us what you need and mail us the vectors. You can create customized gene inserts by ordering gene synthesis, or mail us your template DNA.

GenScript Cloning Services Guide

Service
New! Express Cloning SC1691
Subcloning bundled with
gene synthesis SC1017
Custom PCR Cloning SC1622
Advantages  Free Vectors – Choose from 66 popular mammalian or bacterial expression vectors Get ready to use clones in any vector, commercial or customized  Outsource cloning projects to save time and take the guess work out of recombining templates.  
What you provide 
  • electronic sequence for gene synthesis
  • vector name
  • specify cloning sites if desired
  • electronic sequence for gene synthesis
  • vector details (map) & cloning strategy
  • mail in an aliquot of your vector
  • description of your cloning project needs
  • mail in the DNA component templates.
What you receive
  • 4 μg lyophilized plasmid DNA of your insert in your chosen vector
  • sequence chromatograms, plasmid construct map, and QC data
  • 4 μg lyophilized plasmid DNA of your insert in a standard vector PLUS
  • 4 μg lyophilized plasmid DNA of your insert in your chosen vector
  • sequence chromatograms, plasmid construct map, and QC data
  • 4 μg lyophilized destination vector containing the gene
  • sequence chromatograms, plasmid construct map, and QC data
Starting Price
$49*
$149*
$295
Starting Turnaround Time 
2 days*
5 days*
14 days
How to Order

* Express Cloning and Subcloning bundled with gene synthesis may be combined with Custom Gene Synthesis SC1010 or Fast Gene Synthesis SC1619. If you need cloning for Rush Gene Synthesis, please request a quote. Price and turnaround time listed here are in addition to the price and turnaround time for gene synthesis.

Advantages of Cloning Services from GenScript

By ordering subcloning into your choice of vector, you can receive a ready-to-use construct – not a semi-finished part that will require time-consuming and potentially expensive work to digest, ligate, PCR-amplify, gel purify, transform, screen, prep, and sequence before you can even start your experiments!

  • No more bench work to prepare the construct before you can start your experiments.
  • No more work to order multiple kits and services from multiple vendors to gather all the reagents you need.
  • No more risk of getting the wrong construct, as GenScript ships only 100% sequence-verified constructs with sequence chromatograms.
  • Comparable timeline, but we do the work for you!

To get expression-ready constructs containing a DNA sequence you've designed or that is known from GenBank or other sources, it's usually more efficient to start with 100% sequence verified gene synthesis rather than wasting time trying to clone template DNA that may be of poor quality, truncated, chemically modified or contain contaminats due to  long storage time or prior handling. However, if you need to outsource cloning projects from your template DNA, then GenScript's PCR Cloning services can save you time and take the guess-work out of recombining templates.

Price and Turnaround Time

Gene Insert size+
New! Express Cloning SC1691
Subcloning bundled with gene synthesis SC1017
 
Price
Turnaround Time(business days)
Price
Turnaround Time (business days)
˂3 kb
$49
2
$149
5
3 - 6 kb
$49
2
$199
5
6 -10 kb
$49
2
$249
5
> 10kb
Vectors available* 
Any vector of your choice

+ Express Cloning and Subcloning bundled with gene synthesis may be combined with Custom Gene Synthesis SC1010 or Fast Gene Synthesis SC1619. If you need cloning for Rush Gene Synthesis, please request a quote.

*For Express Cloning, vectors are provided free by GenScript. For Subcloning (SC1017) into vectors that are not on our Express Cloning Vector list, customers must mail an aliquot of the vector to GenScript.

Delivery Specifications

All of our Custom Cloning services guarantee 100% sequence fidelity and deliver everything you need to put your new constructs to work right away. Our delivery package includes the following:

  • 4 μg lyophilized destination vector containing the gene
  • Sequence chromatograms covering your gene (electronic)
  • Construct map for the plasmid (electronic)
  • Quality assurance certificate

Related Services

custom cloning service quotation

Quotations and Ordering

Subcloning For quotation requests and questions, you may contact us by phone, email, fax, or via our secured online quotation system.

Orders can be placed by phone, email, fax, or online with a formal PO (Purchase Order) or credit card.

Please send your samples with full shipping information and PO number (if applicable) with a hard copy of the completed PCR Cloning and Subcloning Quotation Request Form to PCR Cloning and Subcloning Services, GenScript USA Inc., 860 Centennial Avenue., Piscataway, NJ 08854, U.S.

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance.

Note for customers ordering Express Cloning into a pET vector: The pET System allows for expression of target genes under control of the T7 promoter and is covered by US Patent 5,693,489. The patent is licensed by GenScript from Brookhaven National Laboratory. Commercial entities need to obtain a license from Brookhaven National Laboratory prior to use of the custom construct delivered by GenScript.