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*For Non-Clinical Research Use Only*
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DYKDDDDK Peptide  |
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| Full Name |
DYKDDDDK Peptide |
| Alias |
DYKDDDDK tag;Flag-tag |
|
Sequence
(one-letter code) |
DYKDDDDK |
Sequence
(three-letter code) |
{ASP}{TYR}{LYS}{ASP}{ASP}{ASP}{ASP}{LYS} |
| Description |
A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free DYKDDDDK peptide. |
| Purity |
> 95% |
| Storage |
Store at 2-8°C. |
| Notes |
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Related Products
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1 |
Peptide Services: GenScript's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
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2 |
Standard Peptide Synthesis: GenScript offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. GenScript FlexPeptideTM technology can synthesize peptide as long as 200 amino acids, a world record for such technology.
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3 |
Peptide Modifications: GenScript offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
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4 |
Peptide Libraries: GenScript has developed a rapid high-throughput parallel peptide synthesis platform, providing custom libraries of unbound peptides of 5-20 mer at very competitive prices.
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Document-HPLC: 2043_20060721145618.PDF (PDF)