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GSK3α (Phospho-Ser21) PAb Blocking Peptide

Full Name GSK3α (Phospho-Ser21) PAb Blocking Peptide
Alias
Documents
Description
The synthesized phosphopeptide was derived from human GSK3α around the phosphorylation site of serine 21 (T-S-SP-F-A).
MW
0.000
Storage
Store at -20°C
Notes
Concentration
100ug/10 mM phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM N
Quality Control
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Applications

For Western blotting: add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the blot.

For immunohistochemistry: add 2 µl of antibody and 2 µl of blocking peptide to 0.096 ml of buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the slides.

Specificity
The peptide specifically blocks the signal of Rabbit Anti GSK3α (Phospho-Ser21) completely in western blotting and immunohistochemistry.
Related Products
Cat. No.
Product Name
Size
Price
Quantity
A00210-100 GSK3α Antibody (Ab-21), pAb, Rabbit
100 μg
$140.00
A00216-100 GSK3α Antibody (Phospho-Ser21), pAb, Rabbit
100 μg
$188.00
1
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