Home - JP » 製品 » 免疫学製品 » Antibody » peptide : GSK3α (Phospho-Ser21) pAb Blocking Peptide
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  • *This product has been discontinued! *
    GSK3α (Phospho-Ser21) PAb Blocking Peptide

    Full Name GSK3α (Phospho-Ser21) PAb Blocking Peptide
    Description
    The synthesized phosphopeptide was derived from human GSK3α around the phosphorylation site of serine 21 (T-S-SP-F-A).
    Documents
    Document-MSDS: 6154_20081005232937.PDF (PDF)
    TECHNICAL MANUAL: Guidelines_for_Dissolving_Peptides.pdf (pdf)
    Figures
    zoom
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    Reference
    MW
    0.000
    Storage
    Store at -20°C
    Notes
    Concentration
    100ug/10 mM phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM N
    Quality Control
    The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
    Applications

    For Western blotting: add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the blot.

    For immunohistochemistry: add 2 µl of antibody and 2 µl of blocking peptide to 0.096 ml of buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the slides.

    Specificity
    The peptide specifically blocks the signal of Rabbit Anti GSK3α (Phospho-Ser21) completely in western blotting and immunohistochemistry.
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    A00210-100
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    A00216-100
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    1
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