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Cat. No.
Name
Size   
Price
Selected
RP19726
Tau (Phospho-Ser235) pAb Blocking Peptide
100 ?g
$50
Tau (Phospho-Ser235) PAb Blocking Peptide

Full Name Tau (Phospho-Ser235) PAb Blocking Peptide
Description
The synthesized phosphopeptide was derived from human Tau around the phosphorylation site of Serine 235(P-K-SP-P-S).
Documents
Document-MSDS: 6530_20081013041010.PDF (PDF)
TECHNICAL MANUAL: 12464_20100407015038.PDF (PDF)
Figures
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Reference
Storage
Store at -20°C
Notes
Concentration
100ug/10 mM phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM N
Quality Control
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Applications

For Western blotting: add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the blot.

For immunohistochemistry: add 2 µl of antibody and 2 µl of blocking peptide to 0.096 ml of buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the slides.

Specificity
The peptide specifically blocks the signal of Rabbit Anti Tau (Phospho-Ser235) completely in western blotting and immunohistochemistry.
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1
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