Catalog Products » FKHR (Phospho-Ser256) pAb Blocking Peptide
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FKHR (Phospho-Ser256) pAb Blocking Peptide

*This product has been discontinued!*
The synthesized phosphopeptide was derived from human FKHR around the phosphorylation site of serine 256 (A-A-SP-M-D).
RP19739
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Overview
Molecular Weight 0.0
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Properties
Specificity The peptide specifically blocks the signal of Rabbit Anti FKHR (Phospho-Ser256) completely in western blotting and immunohistochemistry.
Concentration 100ug/10 mM phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM N
QC The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Storage Store at -20°C
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Applications

For Western blotting: add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the blot.

For immunohistochemistry: add 2 µl of antibody and 2 µl of blocking peptide to 0.096 ml of buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the slides.
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