Catalog Products » Rb (Phospho-Ser780) pAb Blocking Peptide
Manual
COAs
HPLC
MS

Rb (Phospho-Ser780) pAb Blocking Peptide

*This product has been discontinued!*
The synthesized phosphopeptide was derived from human Rb around the phosphorylation site of Serine 780(T-L-SP-P-I).
RP19769
Ask us a question
Overview
Molecular Weight 0.0
Back

Properties
Specificity The peptide specifically blocks the signal of Rabbit Anti Rb (Phospho-Ser780) completely in western blotting and immunohistochemistry.
Concentration 100ug/10 mM phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM N
QC The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Storage Store at -20°C
Back

Applications

For Western blotting: add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the blot.

For immunohistochemistry: add 2 µl of antibody and 2 µl of blocking peptide to 0.096 ml of buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the slides.
Back