Overlapping Peptide Library :
Overlapping peptide library can be used in epitope mapping. The peptide library generation process is defined by two parameters, peptide length and offset number. The offset number reflects the degree of overlapping. Careful selections of these two parameters are important to achieve optimum balance between data value and the experiment cost. As a general guideline, shorter peptides are easier to synthesize but they have less chances for multiple epitope hits. Greater degree of overlapping (small offset number) gives more chances of multiple epitope hits. Both above stated cases increase the number of peptides to be synthesized for the peptide library.
Used in sequence optimization, Random Library is similar to positional scanning library. It’s constructed by substituting selected positions on the original peptide randomly and simultaneously with all other natural amino acids in a shot gun approach with a purpose to elucidate potential alternatives for enhanced peptide activity.