As one of the pioneers of the life science research service industry, GenScript aggressively seeks to develop new technologies to serve our customers. We have developed and applied our proprietary FlexPeptideTM technology to our peptide-manufacturing pipeline.
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FlexPeptideTM is an advanced integrated approach to peptide synthesis which enables us to secure one of the highest synthesis success rates in the industry, over 95%. This approach is based on the state-of-the-art facility equipped with automated synthesizers for liquid and solid phase peptide synthesis, microwave technologies and proprietary ligation technology. This allows a team of dedicated scientists to develop innovative approaches to synthesize even the most difficult peptide sequences while maintaining a high product yield.
GenScript's FlexPeptideTM technology includes:
- Combined Platforms: flexible choices of LPPS and SPPS, achieved highest synthesis success rate
GenScript can be your reliable partner providing you with customized peptides in a timely fashion. Our extraordinary performance is based on our proprietary FlexPeptideTM technology. With FlexPeptideTM technology, GenScript maintains one of the highest peptide synthesis success rates in the industry, over 95%. We have successfully synthesized multi-cystine peptides with two disulfide bridges.
Case study Ⅰ: Multi-cystine peptides
- Microwave Technologies: synthesize peptides in an efficient and smart way with high yield
With the customizability of the peptides we synthesize in mind, GenScript considers product yield as an important criteria. This is achieved with GenScript's innovated microwave technology. The use of microwave heating in automated peptide synthesis decreases the time required to complete each cycle of coupling and deprotection. The microwave energy delivered keeps the growing peptide chain from folding or forming aggregates and facilitates chemical bonding. These functions allow microwave energy to stitch peptides together easily and efficiently allowing the synthesis of longer peptides and to introduce various modifications to the peptide chains, including addition of sugars and other groups. With microwave technology, we can synthesize phosphopeptides with relative ease.
Case study Ⅱ: Multi-phosphorylated peptides
Microwave Technology Enhances Chemical Coupling in Peptide Chain Assembly
- Ligation Technology: long peptide up to 200 aa can be synthesized
In addition to incorporated microwave technology, FlexPeptideTM employs proprietary ligation methods. These techniques allow us to achieve high yields of very long peptides by first synthesizing several shorter sequences and then ligating them together with high efficiency. GenScript's scientists routinely synthesize peptides of 40-50 aa and can go up to 200 aa without any technical modifications.
Case study Ⅲ: Extremely long peptides
Advantages of GenScript's FlexPeptideTM Technology:
GenScript's peptide synthesis offers researchers the following benefits:
- Guaranteed delivery: Merging LPPS, SPPS, microwave technology and proprietary ligation technology allows us to produce peptides as short as two amino acids or complex sequences of up to 200 amino acids.
- High efficiency: The use of microwave heating in peptide synthesis can markedly reduce the time required to complete each cycle of coupling and deprotection.
- Improved peptide yields: The use of microwave energy can improve peptide yields by preventing unwanted aggregation.
- Decreased cost: Microwave technology significantly decreases the cost of peptide synthesis by enhancing chemical reaction rate and reducing the quantity of chemicals required for synthesis.
- Peptides of any length: Researchers can use proprietary ligation technology and microwave energy to put short peptides together.
- Easier modifications: Microwave energy can also facilitate site-specific peptide modifications.
- Alexander Litovchick and Jack W. Szostak. Selection of cyclic peptide aptamers to HCV IRES RNA using mRNA display. PNAS. Oct 2008; 105(40): 15293 - 15298
- Ke Xu, Iwona Nowak, Michele Kirchner, and Yujia Xu. Recombinant collagen studies link the severe conformational changes induced by osteogenesis imperfecta mutations to the disruption of a set of interchain salt-bridges. J. Biol. Chem. Oct 2008
- Keith W. Wegmann, Cynthia R. Wagner, Ruth H. Whitham, and David J. Hinrichs. Synthetic Peptide Dendrimers Block the Development and Expression of Experimental Allergic Encephalomyelitis. J. Immunol. Sep 2008; 181(5): 3301 - 3309
- Reshma Korde, Ashima Bhardwaj, Rita Singh, Anand Srivastava, Virander S. Chauhan, Raj K. Bhatnagar, Pawan Malhotra. A Prodomain Peptide of Plasmodium falciparum Cysteine Protease (Falcipain-2) Inhibits Malaria Parasite Development. J. Med. Chem. Jun 2008; 51(11): 3116–3123