Phospho Specific AntibodyProtein phosphorylation-dephosphorylation is one of the major signaling mechanisms used to modulate the functional properties of proteins involved in gene expression, cell adhesion, cell proliferation, cell differentiation, and the cell cycle. The determination of the state of phosphorylation of specific proteins is of great value in the pursuit to establish the function of a given protein. Phospho-specific antibodies, which can discriminate between the phosphorylated and non-phosphorylated forms of a given protein, have emerged as demonstrated assets in the study of the signal transduction pathway. These novel tools enable qualitative and quantitative detection of phosphorylated proteins without the risks associated with radioactivity. Phospho-specific antibodies are affinity-purified and depleted for cross-reactivity with non-phosphorylated form of proteins, enabling them to detect specific proteins in the complex mixtures of proteins in cells. They recognize the phosphorylated amino acids in the context of the surrounding amino acid sequence. Hence, using a combination of pan-antibody and phospho-specific antibody, a researcher can assess the degree of phosphorylation of any given protein. GenScript provides most phospho-specific antibodies in pairs, which most researchers find highly convenient. These phospho-specific antibodies are suitable for many tasks, including Western blot analysis, ELISA, and immunocytochemical assays. Categories: |
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