You are here: Biology CRO for Drug Discovery »    Antibody Services » Polyclonal Antibody Services » Custom Polyclonal Antibody Services (Chicken)
GenScript Share

Custom Polyclonal Antibody Services (Chicken)

polyclonal antibody services quotation

Peptide Supplied Polyclonal Antibody Package with IgY purification (Chicken) (Cat. No.: SC1055)

Includes:
Delivery:
Time Frame:
  • Peptide synthesis (<15 a.a., >85% purity, 10.5 mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • Two chickens, pre-immune bleed, four immunizations/chicken
  • IgY Antibody purification
  • ELISA test
  • 2 ml of pre-immune serum*
  • 4 ml of antisera
  • ELISA results
  • Purified IgY antibodies
  • 5 mg of unconjugated peptide, MS and HPLC reports
  • Peptide Synthesis: 2-4 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification and ELISA: 1 week
  • Shipping: 1-2 weeks
  • Total 15-18 weeks

Affinity-Purified Peptide Supplied Polyclonal Antibody Package (Chicken) (Cat. No.: SC1056)

Includes:
Delivery:
Time Frame:
  • Peptide synthesis (<15 a.a, >85% purity, 15.5 mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • Two chickens, pre-immune bleed, four immunizations/chicken
  • Antibody purification by peptide affinity column from yolk
  • Final bleed
  • ELISA test
  • 2 ml of pre-immune serum*
  • 4 ml of antisera
  • ELISA results
  • Affinity-purified IgY antibodies
  • 5 mg of unconjugated peptide, MS and HPLC reports
  • Peptide Synthesis: 2-4 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification and ELISA: 2 weeks
  • Shipping: 1-2 weeks
  • Total 16-19 weeks

* For collection and purification of antibodies from separated animals, a total of 2 ml of pre-immune serum (1 ml from each of the 2 animals) are provided. For antibody purification from pooled serum, 1 ml of pre-immune serum is provided.

Standard Protocol (Two animals)

Steps
Time from
Project Initiation
Time from Immunization
Activity Description
Peptide synthesis
≈2 weeks
-
-
Peptide conjugation to carrier
≈3 weeks
-
-
Control serum collection
≈4 weeks
Day 0
Pre-bleed (2 ml serum)
Primary immunization
≈4 weeks
Day 1
0.1 mg per chicken peptide-KLH conjugate, CFA, s.c.
1st boost
≈6 weeks
Day 14
0.1 mg per chicken peptide-KLH conjugate, IFA, s.c.
Test bleed and ELISA
≈7 weeks
Day 24
Test bleed and ELISA. Evaluation of initial immune response.
2nd boost
≈9 weeks
Day 35
0.1 mg per chicken peptide-KLH conjugate, IFA, s.c.
Antibody Production and ELISA
≈10 weeks
Day 45
Test bleed 1ml per chicken. Egg collection for one week
3rd boost
≈12 weeks
Day 56
0.1 mg per chicken peptide-KLH conjugate, IFA, s.c.
Antibody production
≈13 weeks
Day 63
Egg and bleed collection for one week
ELISA and shipping
≈15 weeks
Day 80
Extend or terminate

Note:

If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products to prevent bacteria growth.

molecuar biology services quotation

For quotation requests and questions, you may contact us by email, phone, fax, or via our secured online quotation system.

Our customer service representatives are available 24 hours Monday through Friday.
You may contact us anytime for assistance.

Email: antibody@genscript.com
Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
Fax: 1-732-210-0262 1-732-885-5878