Custom Polyclonal Antibody Services (Chicken) | | Peptide Supplied Polyclonal Antibody Package with IgY purification (Chicken) (Cat. No.: SC1055) Includes: | Delivery: | Time Frame: | - Peptide synthesis (<15 a.a., >85% purity, 10.5 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Two chickens, pre-immune bleed, four immunizations/chicken
- IgY Antibody purification
- ELISA test
| - 2 ml of pre-immune serum*
- 4 ml of antisera
- ELISA results
- Purified IgY antibodies
- 5 mg of unconjugated peptide, MS and HPLC reports
| - Peptide Synthesis: 2-4 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1 week
- Shipping: 1-2 weeks
- Total 15-18 weeks
| Affinity-Purified Peptide Supplied Polyclonal Antibody Package (Chicken) (Cat. No.: SC1056) Includes: | Delivery: | Time Frame: | - Peptide synthesis (<15 a.a, >85% purity, 15.5 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Two chickens, pre-immune bleed, four immunizations/chicken
- Antibody purification by peptide affinity column from yolk
- Final bleed
- ELISA test
| - 2 ml of pre-immune serum*
- 4 ml of antisera
- ELISA results
- Affinity-purified IgY antibodies
- 5 mg of unconjugated peptide, MS and HPLC reports
| - Peptide Synthesis: 2-4 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 2 weeks
- Shipping: 1-2 weeks
- Total 16-19 weeks
| * For collection and purification of antibodies from separated animals, a total of 2 ml of pre-immune serum (1 ml from each of the 2 animals) are provided. For antibody purification from pooled serum, 1 ml of pre-immune serum is provided. Standard Protocol (Two animals) | Steps | Time from
Project Initiation | Time from Immunization | Activity Description | | Peptide synthesis | ≈2 weeks | | - | | Peptide conjugation to carrier | ≈3 weeks | | - | | Control serum collection | ≈4 weeks | Day 0 | Pre-bleed (2 ml serum) | | Primary immunization | ≈4 weeks | Day 1 | 0.1 mg per chicken peptide-KLH conjugate, CFA, s.c. | | 1st boost | ≈6 weeks | Day 14 | 0.1 mg per chicken peptide-KLH conjugate, IFA, s.c. | | Test bleed and ELISA | ≈7 weeks | Day 24 | Test bleed and ELISA. Evaluation of initial immune response. | | 2nd boost | ≈9 weeks | Day 35 | 0.1 mg per chicken peptide-KLH conjugate, IFA, s.c. | | Antibody Production and ELISA | ≈10 weeks | Day 45 | Test bleed 1ml per chicken. Egg collection for one week | | 3rd boost | ≈12 weeks | Day 56 | 0.1 mg per chicken peptide-KLH conjugate, IFA, s.c. | | Antibody production | ≈13 weeks | Day 63 | Egg and bleed collection for one week | | ELISA and shipping | ≈15 weeks | Day 80 | Extend or terminate | Note: If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products to prevent bacteria growth.   For quotation requests and questions, you may contact us by email, phone, fax, or via our secured online quotation system.
Our customer service representatives are available 24 hours Monday through Friday.
You may contact us anytime for assistance.  | Email: antibody@genscript.com |  | Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |  | Fax: 1-732-210-0262 1-732-885-5878 | |
