Custom Polyclonal Antibody Services (Goat) |
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Complete Peptide Polyclonal Antibody Package (Goat) (Cat. No.: SC1050)
Includes: |
Delivery: |
Time Frame: |
- Peptide synthesis (<15 a.a, >85% purity, 10 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- One goat, pre-immune bleed and pre-screen, four immunization
- Production bleed and final bleed
- ELISA test
- Lyophilization
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- 2 ml of pre-immune serum
- ELISA results
- 1000 ml of antisera (lyophilized) from each goat
- 5 mg of unconjugated peptide, MS and HPLC reports
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- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization and ELISA: 10 weeks
- Lyophilization and Shipping: 1-2 weeks
- Total 14-16 weeks
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Complete Protein G-Purified Peptide Polyclonal Antibody Package (Goat) (Cat. No.: SC1051)
Includes: |
Delivery: |
Time Frame: |
- Peptide synthesis (<15 a.a, >85% purity, 10 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- One goat, pre-immune bleed and pre-screen, four immunization
- Protein G purification of 100 ml antiserum
- Production bleed and final bleed
- ELISA test
- Lyophilization
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- 2 ml of pre-immune serum
- ELISA results
- ≈900 ml antisera
- 300-1,000 mg protein G-purified antibody (lyophilized) from 100 ml antiserum
- 5 mg of unconjugated peptide, MS and HPLC reports
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- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 15-17 weeks
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Complete Affinity-purified Peptide Polyclonal Antibody Package (Goat) (Cat. No.: SC1368)
Includes: |
Delivery: |
Time Frame: |
- Peptide synthesis (<15 a.a, >85% purity, 15 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- One goat, pre-immune bleed and pre-screen, four immunization
- Production bleed and final bleed
- Antigen affinity purification of 100 ml antiserum
- ELISA test
- Lyophilization
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- 2 ml of pre-immune serum
- ELISA results
- ≈900 ml antisera
- 3-5 mg affinity purified antibody (lyophilized) from 100 ml antiserum
- 5 mg of unconjugated peptide, MS and HPLC reports
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- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 15-17 weeks
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Standard Protocol (One goat)
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Steps
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Time from
Project Initiation
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Time from Immunization
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Activity Description
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Peptide synthesis
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≈2 weeks
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Peptide conjugation to carrier
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≈3 weeks
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Control serum collection
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≈4 weeks
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Day 0
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Pre-bleed (≈2 ml serum)
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Primary immunization
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≈4 weeks
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Day 1
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0.8 mg per goat peptide-KLH conjugate, CFA, s.c.
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| 1st boost |
≈6 weeks
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Day 14
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0.8 mg per goat peptide-KLH conjugate, IFA, s.c.
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| Test bleed and ELISA |
≈8 weeks
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Day 21
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2 ml serum per goat. ELISA and evaluation for initial immune response.
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| 2nd boost |
≈9 weeks
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Day 35
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0.8 mg per goat peptide-KLH conjugate, IFA, s.c.
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| Production bleed |
≈11 weeks
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Day 42
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≈250 ml serum per goat
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| 3rd boost |
≈12 weeks
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Day 56
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0.8 mg per goat peptide-KLH conjugate，IFA, s.c.
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| Production bleed |
≈14 weeks
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Day 63
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≈750 ml serum per goat
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| Lyophilization |
≈15 weeks
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Day 70
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| ELISA and shipping |
≈16weeks
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Day 77
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Extend or terminate
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Note:
If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products to prevent bacteria growth. Lyophilization extends the shelf life of your antibodies, eliminates leaking problems and loosens up the stringent temperature requirement during shipping, and reduces the overall weight of antibodies to be shipped.
Quotations and Ordering:
 For quotation requests and questions, you may contact us by email, phone, fax, or via our secured online quotation system.
Our customer service representatives are available 24 hours Monday through Friday.
You may contact us anytime for assistance.
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Email: antibody@genscript.com |
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Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |
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Fax: 1-732-210-0262 1-732-885-5878 |
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