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Custom Polyclonal Antibody Services (Goat)

polyclonal antibody services quotation

Complete Peptide Polyclonal Antibody Package (Goat) (Cat. No.: SC1050)

Includes:
Delivery:
Time Frame:
  • Peptide synthesis (<15 a.a, >85% purity, 10 mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • One goat, pre-immune bleed and pre-screen, four immunization
  • Production bleed and final bleed
  • ELISA test
  • Lyophilization
  • 2 ml of pre-immune serum
  • ELISA results
  • 1000 ml of antisera (lyophilized) from each goat
  • 5 mg of unconjugated peptide, MS and HPLC reports
  • Peptide Synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization and ELISA: 10 weeks
  • Lyophilization and Shipping: 1-2 weeks
  • Total 14-16 weeks

Complete Protein G-Purified Peptide Polyclonal Antibody Package (Goat) (Cat. No.: SC1051)

Includes:
Delivery:
Time Frame:
  • Peptide synthesis (<15 a.a, >85% purity, 10 mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • One goat, pre-immune bleed and pre-screen, four immunization
  • Protein G purification of 100 ml antiserum
  • Production bleed and final bleed
  • ELISA test
  • Lyophilization
  • 2 ml of pre-immune serum
  • ELISA results
  • ≈900 ml antisera
  • 300-1,000 mg protein G-purified antibody (lyophilized) from 100 ml antiserum
  • 5 mg of unconjugated peptide, MS and HPLC reports
  • Peptide Synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification and ELISA: 1 week
  • Lyophilization and Shipping: 1-2 weeks
  • Total 15-17 weeks

Complete Affinity-purified Peptide Polyclonal Antibody Package (Goat) (Cat. No.: SC1368)

Includes:
Delivery:
Time Frame:
  • Peptide synthesis (<15 a.a, >85% purity, 15 mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • One goat, pre-immune bleed and pre-screen, four immunization
  • Production bleed and final bleed
  • Antigen affinity purification of 100 ml antiserum
  • ELISA test
  • Lyophilization
  • 2 ml of pre-immune serum
  • ELISA results
  • ≈900 ml antisera
  • 3-5 mg affinity purified antibody (lyophilized) from 100 ml antiserum
  • 5 mg of unconjugated peptide, MS and HPLC reports
  • Peptide Synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification and ELISA: 1 week
  • Lyophilization and Shipping: 1-2 weeks
  • Total 15-17 weeks

Standard Protocol (One goat)

Steps
Time from
Project Initiation
Time from Immunization
Activity Description
Peptide synthesis
≈2 weeks
-
-
Peptide conjugation to carrier
≈3 weeks
-
-
Control serum collection
≈4 weeks
Day 0
Pre-bleed (≈2 ml serum)
Primary immunization
≈4 weeks
Day 1
0.8 mg per goat peptide-KLH conjugate, CFA, s.c.
1st boost
≈6 weeks
Day 14
0.8 mg per goat peptide-KLH conjugate, IFA, s.c.
Test bleed and ELISA
≈8 weeks
Day 21
2 ml serum per goat. ELISA and evaluation for initial immune response.
2nd boost
≈9 weeks
Day 35
0.8 mg per goat peptide-KLH conjugate, IFA, s.c.
Production bleed
≈11 weeks
Day 42
≈250 ml serum per goat
3rd boost
≈12 weeks
Day 56
0.8 mg per goat peptide-KLH conjugate,IFA, s.c.
Production bleed
≈14 weeks
Day 63
≈750 ml serum per goat
Lyophilization
≈15 weeks
Day 70
-
ELISA and shipping
≈16weeks
Day 77
Extend or terminate

Note:

If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products to prevent bacteria growth. Lyophilization extends the shelf life of your antibodies, eliminates leaking problems and loosens up the stringent temperature requirement during shipping, and reduces the overall weight of antibodies to be shipped.

Quotations and Ordering:

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