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HEPES

Catalog No.	Name	Group	Cas	Price
				100 g	500 g	5000 g	25000 g
C01621	HEPES	Biological Buffers	7365-45-9	$25.60	$89.60	$627.20	$2195.20

Description:

HEPES is a widely used buffer in biological studies. There is only one restriction in the use of this buffer, because it interferes with the Folin protein assay. In cell culture media, it is employed as a substitute for the bicarbonate buffer at a concentration of 25 mM or as a supplement to the bicarbonate buffer (concentration 10-15 mM). The addition of 20 mM HEPES to TBE buffer improves the migration behaviour of certain samples in the SSCP analysis (single-strand conformation polymorphism), with a higher resolution especially if small differences in the sequences between certain bands are wanted.

Synonyms:

4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid; N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid); N-(2-Hydroxyethyl)piperazine-N'-ethanesulfonic Acid

Molecular Formula:

C₈H₁₈N₂O₄S

Molecular Weight:

238.30

Appearance:

White crystalline powder

Useful pH Range:

pH 6.8-8.2

pKa:

7.55 (25°C)

Storage:

Room temperature

Specification:

Assay	≥99% (titr.)
pH	4.7-6.0 (1%, H2O)
Moisture	≤0.5%
Heavy Metals	≤5 PPM (as Pb)

STRUCTURE:

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DATASHEET:  20080625042850  (PDF)

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	Code	Name	Size	Price	Quantity
	C01621-100 g	HEPES	100 g	$25.60
	C01621-500 g	HEPES	500 g	$89.60
	C01621-5000 g	HEPES	5000 g	$627.20
	C01621-25000 g	HEPES	25000 g	$2195.20