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Taq DNA Polymerase


Key Feature

Description
Taq DNA Polymerase is a thermostable DNA Polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used for PCR*.

  • Note: This product is supplied with 10X reaction buffer containing 15 mM magnesium chloride. dNTP (10 mM) mixture must be ordered separately (see related products).

    Applications
    The applications of Taq DNA Polymerase include the following:
    • PCR*
    • 3'A-tailing of blunt ends
    • Primer extension
    • DNA sequencing


    Formulation
    GenScript Taq DNA Polymerase has been formulated using GenScript's proprietary technology. The enzyme can be shipped at room temperature or even 37°C for seven days without any loss of activity.
    QC Tests
    PCR* performance, activity, nuclease
    Lane Taq Unit
    1Leading
    Brand A    		0.1
    2 0.25
    3 0.5
    4Leading
    Brand B    		0.1
    5 0.25
    6 0.5
    7GenScript0.1
    8 0.25
    9 0.5

    Unit Definition
    One unit is defined as the amount of enzyme that can incorporates 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
    10 X Reaction Buffer (with Mg2+)
    500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100
    Buffer. This buffer is optimized for use with 200 µM dNTPs.
    Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity.
    Concentration:
    Taq is delivered in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.
    Storage
    Store the product at -20°C.

    * The PCR process is covered by US. Patents Nos. 4683195 and 4683202, issued to Cetus and owned by Hoffman-La Roche Inc. GenScript does not encourage or support the unauthorized use of the PCR process. Use of this product is recommended for persons who either have a license to perform PCR or are not required to obtain a license. Sale of this product is restricted to regions or countries where native Taq DNA polymerase patents have been invalidated.

    DATASHEET:  20060806230259  (PDF)
    MSDS:  20060807040514  (PDF)
    PROTOCOL:  20061215120639  (PDF)
    COA:  E00007_R201905  (PDF)
    COA:  E00007_R206904  (PDF)
    COA:  E00007_R204907  (PDF)
    COA:  E00007_R217906  (PDF)
    COA:  E00007_R219906  (PDF)
    COA:  E00007_R206910  (PDF)
    COA:  E00007_R207909  (PDF)
    COA:  E00007_R20011002  (PDF)
    COA:  E00007_R20031003  (PDF)
    COA:  E00007_R60011007  (PDF)
    COA:  E00007_R60011010  (PDF)
    COA:  E00007_R60011101  (PDF)
    COA:  E00007_R60071104  (PDF)
    COA:  E00007_C20031110  (PDF)
    COA:  E00007_C20011109  (PDF)

    Publications used this GenScript Product:
    • Gartner W, Vila G, Daneva T, Nabokikh A, Koc-Saral F, Ilhan A, Majdic O, Luger A, Wagner L. New functional aspects of the neuroendocrine marker secretagogin based on the characterization of its rat homologue. Am J Physiol Endocrinol Metab. Apr 2007; 293: E347-E354

    • Abajy MY, Kopec J, Schiwon K, Burzynski M, Doring M, Bohn C, Grohmann E. A Type IV-Secretion-Like System (T4SLS) is Required for Conjugative DNA Transport of Plasmid pIP501 with Broad Host Range in Gram-Positive Bacteria. J. Bacteriol. Jan 2007; 189(6): 2487-2496

    • Zeyu Xin, Yihong Zhao, and Zhi-Liang Zheng. Transcriptome Analysis Reveals Specific Modulation of Abscisic Acid Signaling by ROP10 Small GTPase in Arabidopsis. Plant Physiol. Oct 2005; 139(3): 1350-1365

    • Gerard Crudden, Rachel E. Chitti, and Rolf J Craven. Hpr6 (heme-1 domain protein) regulates the susceptibility of cancer cells to chemotherapeutic drugs. J. Pharmacol. Exp. Ther. Oct 2005; 316(1): 448-455

    • Julia C Mallory, Gerard Crudden, Amelia Oliva, Christopher Saunders, Arnold Stromberg, Rolf J Craven. A novel group of genes regulate susceptibility to anti-neoplastic drugs in highly tumorigenic breast cancer cells. Mol. Pharmacol. Sep 2005; 68(6): 1747-1756


    CodeNameSizePriceQuantity
    E00007-1000 UTaq DNA Polymerase1000 U$60.00
    E00007-50000 UTaq DNA Polymerase50000 U$2700.00
    E00100Complete Taq system: 1000 U of Taq (E00007); 0.5 ml of 10 mM dNTP (D0056); 100 loading of DNA maker (M103R)$144.00
    E00101Taq and dNTP: 1000 U of Taq (E00007); 0.5 ml of 10 mM dNTP (D0056)$78.00


    Related Products:

     CodeNameSizePriceQuantity
    E00043Enzyme : Green Taq DNA Polymerase 1000 U
    $ 60.00
    E00008Enzyme : Taq DNA Polymerase without Mg2+ 1000 U
    $ 60.00
    E00101Taq and dNTP: 1000 U of Taq (E00007); 0.5 ml of 10 mM dNTP (D0056) 1 PK
    $ 78.00
    D0056dNTP mixture, 10 mM each 0.5 ml
    $ 25.00


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