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- Supplied with 10X reaction buffer containing 15 mM magnesium chloride. dNTP (10 mM) mixture must be ordered separately (see related products).
Description: Taq DNA Polymerase is a thermostable DNA Polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used for PCR*.
Applications: The applications of Taq DNA Polymerase include the following:
- PCR*
- 3'A-tailing of blunt ends
- Primer extension
- DNA sequencing
Formulation: GenScript Taq DNA Polymerase has been formulated using GenScript's proprietary technology. The enzyme can be shipped at room temperature or even 37°C for seven days without any loss of activity.
Features:
- Terminal transferase activity: Taq DNA Polymerase has terminal transferase activity which results in the addition of a single nucleotide (adenosine) at the 3' end of the extension product.
- High-purity: No contamination activity has been detected in standard test reactions.
QC Tests: PCR* performance, activity, nuclease
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Unit Definition: One unit is defined as the amount of enzyme that can incorporates 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
10 X Reaction Buffer (with Mg2+): 500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100
Buffer. This buffer is optimized for use with 200 µM dNTPs.
Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity.
Storage Buffer and Concentration: Taq is delivered in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.
Storage: Store the product at -20°C.
*The PCR process is covered by US. Patents Nos. 4683195 and 4683202, issued to Cetus and owned by Hoffman-La Roche Inc. GenScript does not encourage or support the unauthorized use of the PCR process. Use of this product is recommended for persons who either have a license to perform PCR or are not required to obtain a license. Sale of this product is restricted to regions or countries where native Taq DNA polymerase patents have been invalidated.
MSDS: 20060807040514 (PDF)
PROTOCOL: 20061215120639 (PDF)
Publications used this GenScript Product:
- Gartner W, Vila G, Daneva T, Nabokikh A, Koc-Saral F, Ilhan A, Majdic O, Luger A, Wagner L. New functional aspects of the neuroendocrine marker secretagogin based on the characterization of its rat homologue. Am J Physiol Endocrinol Metab. Apr 2007; 293: E347-E354
- Abajy MY, Kopec J, Schiwon K, Burzynski M, Doring M, Bohn C, Grohmann E. A Type IV-Secretion-Like System (T4SLS) is Required for Conjugative DNA Transport of Plasmid pIP501 with Broad Host Range in Gram-Positive Bacteria. J. Bacteriol. Jan 2007; 189(6): 2487-2496
- Zeyu Xin, Yihong Zhao, and Zhi-Liang Zheng. Transcriptome Analysis Reveals Specific Modulation of Abscisic Acid Signaling by ROP10 Small GTPase in Arabidopsis. Plant Physiol. Oct 2005; 139(3): 1350-1365
- Gerard Crudden, Rachel E. Chitti, and Rolf J Craven. Hpr6 (heme-1 domain protein) regulates the susceptibility of cancer cells to chemotherapeutic drugs. J. Pharmacol. Exp. Ther. Oct 2005; 316(1): 448-455
- Julia C Mallory, Gerard Crudden, Amelia Oliva, Christopher Saunders, Arnold Stromberg, Rolf J Craven. A novel group of genes regulate susceptibility to anti-neoplastic drugs in highly tumorigenic breast cancer cells. Mol. Pharmacol. Sep 2005; 68(6): 1747-1756