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Description: Taq DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA polymerase is the most common polymerase used for PCR^*.

Applications: The applications of Taq DNA Polymerase include the following:

• PCR^*
• 3' A-tailing of blunt ends
• Primer extension
• DNA sequencing

Formulation: GenScript Taq DNA Polymerase has been formulated using GenScript's proprietary technology. The enzyme can be shipped at room temperature or even 37°C for seven days without any loss of activity.

Features:

• Terminal transferase activity: Taq DNA Polymerase has terminal transferase activity, which results in the addition of a single nucleotide (adenosine) at the 3' end of the extension product.
• High purity: No contamination activity has been detected in standard test reactions.

QC Tests: PCR^* performance, activity, nuclease

Lane Taq Unit used 1 Leading Brand A 0.1 2 0.25 3 0.5 4 Leading Brand B 0.1 5 0.25 6 0.5 7 GenScript 0.1 8 0.25 9 0.5

Unit Definition: One unit is the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

10 X Reaction Buffer (without Mg²⁺): 500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 1% Triton X-100 Buffer. This buffer is optimized for use with 200 µM dNTPs.
Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity.

Storage Buffer and Concentration: Taq is delivered in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.

Storage: -20 °C

*The PCR process is covered by US. Patents numbers 4683195 and 4683202, issued to Cetus and owned by Hoffman-La Roche Inc. GenScript does not encourage or support the unauthorized use of the PCR process. Use of this product is recommended for persons who either have a license to perform PCR or are not required to obtain a license. Sale of this product is restricted to regions or countries where native Taq DNA polymerase patents have been invalidated. Store at -20°C

MSDS:  20080228031657  (PDF)