Description:
GenScript's GCpro Taq DNA Polymerase is a versatile and easy-to use enzyme, with powerful advantages for all PCR applications. GCpro Taq DNA polymerase is prepared from a recombinant clone expressed in E. coli, containing the DNA polymerase I gene from Thermococcus litoralis. The enzyme includes a highly processive 5'-3' DNA polymerase activity. Its inherent 3'-5' proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq DNA Polymerase. The enzyme includes an optimized reaction buffer, which enables the amplification of GC-rich templates and templates with problematic secondary structures that would otherwise interfere with the PCR process. GCpro Taq DNA Polymerase provides robust synthesis of longer GC-rich amplification products. GCpro Taq DNA polymerase has all the properties of regular Taq DNA Polymerases for every standard PCR application. In additionm, it is versatile, user-friendly and compatible with all PCR applications, even the amplification of GC-rich and other complex templates, site-directed mutagenesis and TA cloning.
Applications:
GCpro Taq DNA Polymerase can be used in many applications including the following:
- Excellent yields with GC-rich and other complex templates
- Site-directed mutagenesis
- Higher fidelity than standard Taq DNA polymerase
- Cloning with TA end
- High specific activity
- High specificity due to combination of enzyme and unique PCR buffer
1. High yields with GC rich template of Chr19 human genomic DNA:
2. Amplification:
Contents:
- GCpro Taq DNA Polymerase
- 1.5 ml×2 2X buffer, 500 mM Tris-HCl (pH8.9 at 25°C), 0.5% Tween 20, 0.5% Nonidet P40, 1 mg/ml BSA, with 3 mM MgCl2
Activity:
GCpro Taq DNA Polymerase shows very good fidelity and an average error rate around 2X10-6.
GCpro Taq DNA Polymerase has no 5'->3' exonuclease activity. It also has the extendase activity necessary to TA cloning.
Unit Definition:
One unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
Storage Buffer and Concentration:
Supplied in 5 units/µl in 50 mM Tris-HCl (pH8.1), 0.1 mM EDTA, 1 mM DTT, 0.1% (v/v) Nonidet P40, 0.1% (v/v) Tween 20, and 50% (v/v) glycerol.
Storage:
Store the enzyme at -20°C. It will remain stable for at least one year if stored properly.
MSDS: 20060919222614 (PDF)
PROTOCOL: 20060919235411 (DOC)
PROTOCOL: 20060919235455 (PDF)
