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Description: Taq DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used in PCR*. In some cases, such as RAPD PCR, adding large volume of general Taq DNA polymerase (5 U/μl), which has a high concentration of glycerol in its storage buffer, will increase the glycerol concentration in the reaction mix, interfering with PCR performance. The use of concentrated Taq DNA Polymerase (25 U/μl ), with a far slimmer dose of glycerol, can prevent poor PCR efficiency. Applications: The applications of Taq DNA polymerase are as follows:
Formulation: Genscript Taq DNA Polymerase has been formulated using GenScript's proprietary technology. The enzyme can be shipped at room temperature or stored at 37°C for seven days without any significant loss of activity. Features:
QC Tests: PCR* performance, activity, nuclease
Unit Definition: one unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. 10 X Reaction Buffer (with Mg2+): 500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100 Buffer. This buffer is optimized for use with 200 µM dNTPs. Storage Buffer and Concentration: Supplied in 25 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol. Storage: Store the product at -20°C.
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