• GenScript Taq DNA Polymerase is supplied with 10X reaction buffer containing 15 mM magnesium chloride. The dNTP (10 mM) mixture may be ordered separately (See related products).

Description: Taq DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used in PCR^*. In some cases, such as RAPD PCR, adding large volume of general Taq DNA polymerase (5 U/μl), which has a high concentration of glycerol in its storage buffer, will increase the glycerol concentration in the reaction mix, interfering with PCR performance. The use of concentrated Taq DNA Polymerase (25 U/μl ), with a far slimmer dose of glycerol, can prevent poor PCR efficiency.

Applications: The applications of Taq DNA polymerase are as follows:

• PCR^*
• 3"A-tailing of blunt ends
• Primer extension
• DNA sequencing

Formulation: Genscript Taq DNA Polymerase has been formulated using GenScript's proprietary technology. The enzyme can be shipped at room temperature or stored at 37°C for seven days without any significant loss of activity.

Features:

• Terminal transferase activity: A single nucleotide (adenosine) is added to the 3’ end of the extension product.
• High-purity: No contamination activity has been detected in standard test reactions.

QC Tests: PCR^* performance, activity, nuclease

Lane Taq Amount Used 1 Leading Brand A 0.1 2 0.25 3 0.5 4 Leading Brand B 0.1 5 0.25 6 0.5 7 GenScript 0.1 8 0.25 9 0.5

Unit Definition: one unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

10 X Reaction Buffer (with Mg2+): 500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl₂, 1% Triton X-100 Buffer. This buffer is optimized for use with 200 µM dNTPs.
Note: 0.1% Triton X-100 (final concentration) is a must to ensure high activity. GenScript Triton X-100 Buffer is provided ready-to-use, but GenScript Taq DNA Polymerase is compatible with other buffers.

Storage Buffer and Concentration: Supplied in 25 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.

Storage: Store the product at -20°C.

PROTOCOL:  20070118041907  (PDF)
MSDS:  20080228032114  (PDF)