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Products » Molecular Biology Products » PCR Reagents » Enzyme : Taq DNA Polymerase, concentrated
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Description:
Taq DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used in PCR*. In some cases, such as RAPD PCR, adding large volume of general Taq DNA polymerase (5 U/μl), which has a high concentration of glycerol in its storage buffer, will increase the glycerol concentration in the reaction mix, interfering with PCR performance. The use of concentrated Taq DNA Polymerase (25 U/μl ), with a far slimmer dose of glycerol, can prevent poor PCR efficiency.
Applications:
The applications of Taq DNA polymerase are as follows:
Formulation:
Genscript Taq DNA Polymerase has been formulated using GenScript's proprietary technology. The enzyme can be shipped at room temperature or stored at 37°C for seven days without any significant loss of activity.
QC Tests:
PCR* performance, activity, nuclease
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Unit Definition:
one unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
10 X Reaction Buffer (with Mg2+):
500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100 Buffer. This buffer is optimized for use with 200 µM dNTPs.
Important: If another reaction buffers are used with Taq DNA Polymerase, Triton X-100 must be added to a final concentration of 0.1% to ensure high enzyme activity with Taq DNA Polymerase;
Concentration:
Supplied in 25 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.
Storage: Store the product at -20°C.
PROTOCOL: 20070118041907 (PDF)
MSDS: 20080228032114 (PDF)







