<i>Taq</i> DNA Polymerase, concentrated

You are here: Products » Molecular Biology Products » PCR Reagents » Enzyme : Taq DNA Polymerase, concentrated

Key Features:

Terminal Transferase Activity: A single nucleotide (adenosine) is added to the 3′ end of the extension product.

High-purity: No contamination activity has been detected in standard test reactions.

Description:
Taq DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used in PCR^*. In some cases, such as RAPD PCR, adding large volume of general Taq DNA polymerase (5 U/μl), which has a high concentration of glycerol in its storage buffer, will increase the glycerol concentration in the reaction mix, interfering with PCR performance. The use of concentrated Taq DNA Polymerase (25 U/μl ), with a far slimmer dose of glycerol, can prevent poor PCR efficiency.

Note: Concentrated Taq DNA Polymerase (GenScript, E00012) is supplied with 10X reaction buffer containing 15 mM magnesium chloride. The dNTP (10 mM) mixture may be ordered separately (See related products).

Applications:
The applications of Taq DNA polymerase are as follows:

PCR^*

3'A-tailing of blunt ends

Primer extension

DNA sequencing

Formulation:
Genscript Taq DNA Polymerase has been formulated using GenScript's proprietary technology. The enzyme can be shipped at room temperature or stored at 37�C for seven days without any significant loss of activity.

QC Tests:
PCR^* performance, activity, nuclease

Lane	Taq	Amount Used
1	Leading Brand A	0.1
2		0.25
3		0.5
4	Leading Brand B	0.1
5		0.25
6		0.5
7	GenScript	0.1
8		0.25
9		0.5

Unit Definition:
one unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74�C.

10 X Reaction Buffer (with Mg²⁺):
500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25�C), 15 mM MgCl₂, 1% Triton X-100 Buffer. This buffer is optimized for use with 200 �M dNTPs.

Important: If another reaction buffers are used with Taq DNA Polymerase, Triton X-100 must be added to a final concentration of 0.1% to ensure high enzyme activity with Taq DNA Polymerase;

Concentration:
Supplied in 25 units/�l in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.

Storage: Store the product at -20�C.

PROTOCOL: 20070118041907 (PDF)
MSDS: 20080228032114 (PDF)