Increased stability: LaPro Taq DNA Polymerase is more stable than standard Taq DNA polymerase.Applications:
Some of the applications of LApro Taq DNA Polymerase are as follows:
- Up to 20kb from λDNA
- High fidelity
- More stable than normal Taq DNA Polymerase
- TA cloning
- Site-directed mutagenesis
- Fragment analysis
Contents:
- LApro Taq DNA Polymerase
- 1.5ml×2 2x buffer, 500 mM Tris-HCl (pH 8.9 at 25°C), 0.5 % Tween 20, 0.5% Nonidet P40, 1 mg/ml BSA ,with 3 mM MgCl2
- dNTP,10 mM 50ul
- Control template and primers for 25 reactions
Activity:
LApro Taq DNA Polymerase shows very good fidelity and an average error rate around 2x10-6. LApro Taq DNA Polymerase has 5'->3' exonuclease activity and 3'->5' proofreading activity. It also has the extendase activity allowing TA cloning.
Unit Definition:
One unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
Storage Buffer and Concentration:
Supplied in 5 units/µl in 50 mM Tris HCl (pH 8.1), 0.1 mM EDTA, 1 mM DTT, 0.1% (v/v) Nonidet P40, 0.1% (v/v) Tween 20 and 50% (v/v) glycerol.
Storage:
Store at -20°C. The enzyme will remain stable for at least one year, if stored properly.
PROTOCOL: 20061128220256 (PDF)
MSDS: 20080228032444 (PDF)
