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Description:
This kit contains all the reagents required for a complete PCR, combining simple handling with high flexibility. Its premium polymerase, ultrapure dNTPs, and optimized complete reaction buffer ensure superior amplification results. This detergent-free buffer is designed for applications such as DHPLC, which benifit from PCR products that are free of detergents. They can also be used in microarray applications, since detergents can cause troublesome foaming when PCR products are spotted on microarray slides. The kit is recommended for use with routine PCR. It is optimized for high specificity and minimal by-product formation. The buffer system is particularly suitable for plate-based PCR and automated pipetting where a detergent-free buffer system is required.

Key Features:

• All components, including the Taq DNA Polymerase and PCR buffer, are completely detergent-free.
• PCR products are immediately ready for HPLC purification without any further treatment.
• Our specialized detergent-free buffer system is ideal for high-throughput PCR and HPLC.

Contents:

• Hi-Thru Taq DNA Polymerase
• 10X Taq buffer without detergent: 100 mM Tris-HCl, 500 mM KCl, 15 mM MgCl₂, pH8.3 at 25°C
• dNTP Mixture, 10 mM each, 200 µl

Activity:
The enzyme replicates DNA at 72°C. In the presence of magnesium, it catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction. It also demonstrates extendase activity, allowing TA cloning.

Unit Definition:
One unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

Storage Buffer and Concentration:
Supplied in 5 units/µl in 20 mM Tris-HCl, 100 mM KCl, 0.1 EDTA, 1 mM DTT, 50% (v/v) glycerol, pH8.0 (25°C).

Storage:
Quality is guaranteed for 12 months. Store the product at -20°C. Avoid frequent thawing and freezing.

PROTOCOL:  20070121215553  (PDF)
MSDS:  20080228032547  (PDF)